Anti-ERCC6 Polyclonal Antibody for WB, IHC-P, ELISA - Q03468

Core Product Specifications and Parameters

Biological Background: ERCC6 Function and Localization

• DNA excision repair protein ERCC-6 (also known as CSB) functions as an essential ATP-dependent helicase in transcription-coupled nucleotide excision repair (TC-NER), recognizing stalled RNA polymerase II and recruiting ERCC8/CSA and TFIIH to initiate lesion removal. Related references: PMID:16246722 PMID:20541997 PMID:22483866 PMID:32355176 PMID:34526721 PMID:38600235
• Upon DNA binding, ERCC6 wraps DNA around itself, locally modifying chromatin conformation and the interface between RNA polymerase II and damaged DNA. Related references: PMID:15548521
• Acts as a chromatin remodeler at DNA double-strand breaks (DSBs), utilizing its DNA-dependent ATPase activity to evict histones from flanking chromatin, facilitating repair factor access. Related references: PMID:16246722 PMID:9565609 PMID:29203878
• Regulates DSB repair pathway choice by inhibiting non-homologous end joining (NHEJ) and promoting homologous recombination (HR); also mediates ATM/CHEK2-dependent checkpoint activation to prevent premature mitosis. Related references: PMID:25820262
• Required for neuronal differentiation and neuritogenesis through its transcription and chromatin remodeling activities during neurogenesis. Related references: PMID:24874740
• Localizes primarily to the nucleus and associates with chromosomes; implicated in Cockayne syndrome, UV-sensitive syndrome, and age-related macular degeneration.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 700–800 of human ERCC6, targeting a central region likely conserved across isoforms; verify specificity in your sample system if multiple isoforms are present.
• For Western blot, anticipate a band around 168 kDa. Use a low-percentage gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), nuclear ERCC6 detection may require antigen retrieval optimization; include positive control tissues (e.g., testis, proliferating cells) and a negative control (e.g., knockout material if available) to confirm staining specificity.
• Cross-reactivity with mouse and rat has been observed; validate the antibody in the relevant species and tissue context before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development from concept to clinic. This anti-ERCC6 polyclonal antibody is well-suited for research in DNA repair, chromatin dynamics, and related disease mechanisms, and CamelBio can further assist with sourcing rare-target raw materials and offering technical consulting tailored to your assay requirements.