Anti-Emerin/EMD Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50402

Core Product Specifications and Parameters

Biological Background: Emerin Function and Localization

• Emerin/EMD is a 29 kDa type II integral membrane protein of the nuclear envelope, encoded by the EMD gene (aliases STA, EDMD, LEMD5; UniProt P50402).
• Stabilizes the nuclear actin cortical network and stimulates actin polymerization by binding the pointed end of actin filaments (PubMed:15328537). Related references: PMID:15328537
• Acts as an inhibitor of beta-catenin activity by facilitating its nuclear export via a CRM1-dependent pathway (PubMed:16858403). Related references: PMID:16858403
• Links centrosomes to the nuclear envelope through microtubule association (PubMed:17785515). Related references: PMID:17785515
• Required for proper localization of non-farnesylated prelamin-A/C, important for nuclear lamina architecture (PubMed:19323649). Related references: PMID:19323649
• Cooperates with NEMP1 to maintain nuclear envelope stiffness in germ cells (PubMed:32923640). Related references: PMID:32923640
• Localizes to both the inner and outer nuclear membranes; highly expressed in skeletal muscle, heart, colon, testis, ovary, and pancreas, and is implicated in Emery-Dreifuss muscular dystrophy.

Experimental Guidance and Technical Tips

• Immunogen design covers the N-terminal 220 amino acids of human Emerin, targeting the nucleoplasmic region; the antibody may detect full-length endogenous protein and potential isoforms.
• For Western blot (WB), reduce samples and load 20–30 µg of total protein; expect a band at approximately 29 kDa in human and mouse tissue lysates.
• In immunohistochemistry (IHC-P), antigen retrieval and permeabilization may be required for nuclear envelope labeling; validate in tissues with high Emerin expression (e.g., skeletal muscle, heart).
• For immunofluorescence (IF/ICC), fix cells with paraformaldehyde and permeabilize with Triton X-100; Emerin shows a characteristic nuclear rim pattern.
• ELISA and other applications should be optimized in the relevant sample system; consider using known positive controls (e.g., HeLa, C2C12).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated IVD raw-material sourcing partner, offering this high-quality anti-Emerin polyclonal antibody alongside a comprehensive portfolio of validated antibody pairs, monoclonals, and ancillary reagents for nuclear envelope and muscular dystrophy research. With expert technical support and flexible supply, we bridge the gap from discovery to diagnostic development.