Anti-Emerin/EMD Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50402

Core Product Specifications and Parameters

Biological Background: Emerin Function and Localization

• Emerin, encoded by the EMD gene (aliases STA, EDMD, LEMD5), is a nuclear envelope protein.
• It stabilizes and promotes the formation of a nuclear actin cortical network by binding and stabilizing the pointed end of growing actin filaments.
  Related references: PMID:15328537
• It inhibits beta-catenin activity by preventing its nuclear accumulation through a CRM1-dependent export pathway.
  Related references: PMID:16858403
• It links centrosomes to the nuclear envelope via a microtubule association.
  Related references: PMID:17785515
• It is required for proper localization of non-farnesylated prelamin-A/C.
  Related references: PMID:19323649
• Together with NEMP1, it contributes to nuclear envelope stiffness in germ cells.
  Related references: PMID:32923640
• It localizes to the nucleus inner and outer membrane, and is expressed in skeletal muscle, heart, colon, testis, ovary, and pancreas. Associated with Emery-Dreifuss muscular dystrophy and cardiomyopathy; subject to acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-220) covers the conserved N-terminal region; consider that this may contribute to the observed mouse cross-reactivity.
• For Western blotting, the predicted molecular weight is ~29 kDa; validate band size in relevant cell or tissue lysates, considering possible post-translational modifications.
• For IHC-P and IF/ICC, optimize fixation and permeabilization protocols, as Emerin is a nuclear envelope protein; antigen retrieval may be required.
• ELISA detection may be used for native or recombinant Emerin; consider coating buffer and blocking conditions to maintain protein conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined one-stop sourcing platform for IVD raw materials, from early-stage development to clinical application. This Emerin/EMD antibody supports studies on nuclear envelope dynamics and Emery-Dreifuss muscular dystrophy, and can be complemented by our validated antibody pairs, optimized monoclonal/polyclonal reagents, and ancillary raw-material supply services. For rare-target or custom sourcing needs, our team bridges the gap with efficient procurement and technical consultation.