Polyclonal Antibodies
Anti-Emerin/EMD Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50402
Item Number : CM0016561
Price varies based on specs and customizations
- Application
- WB, IHC-P, IF/ICC, ELISA
- Cross Reactivity
- Human, Mouse
- Protein Weight
- 29kDa
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Core Product Specifications and Parameters
| Parameter | Value |
|---|---|
| Product Name | Emerin/EMD Rabbit pAb |
| Remarks/Alias | STA; EDMD; LEMD5; Emerin/EMD |
| Species | Human |
| GeneID | 2010 |
| Immunogen | Recombinant protein: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Emerin/EMD (NP_000108.1). |
| Source | Rabbit |
| Category | Polyclonal Antibodies |
| Application | WB, IHC-P, IF/ICC, ELISA |
| Cross Reactivity | Human, Mouse |
| SWISS | P50402 |
| Protein Weight | 29kDa |
| Shipping | Ice bag |
Biological Background: Emerin Function and Localization
- Emerin, encoded by the EMD gene (aliases STA, EDMD, LEMD5), is a nuclear envelope protein.
- It stabilizes and promotes the formation of a nuclear actin cortical network by binding and stabilizing the pointed end of growing actin filaments.
Related references: PMID:15328537 - It inhibits beta-catenin activity by preventing its nuclear accumulation through a CRM1-dependent export pathway.
Related references: PMID:16858403 - It links centrosomes to the nuclear envelope via a microtubule association.
Related references: PMID:17785515 - It is required for proper localization of non-farnesylated prelamin-A/C.
Related references: PMID:19323649 - Together with NEMP1, it contributes to nuclear envelope stiffness in germ cells.
Related references: PMID:32923640 - It localizes to the nucleus inner and outer membrane, and is expressed in skeletal muscle, heart, colon, testis, ovary, and pancreas. Associated with Emery-Dreifuss muscular dystrophy and cardiomyopathy; subject to acetylation and phosphorylation.
Experimental Guidance and Technical Tips
- The immunogen (aa 1-220) covers the conserved N-terminal region; consider that this may contribute to the observed mouse cross-reactivity.
- For Western blotting, the predicted molecular weight is ~29 kDa; validate band size in relevant cell or tissue lysates, considering possible post-translational modifications.
- For IHC-P and IF/ICC, optimize fixation and permeabilization protocols, as Emerin is a nuclear envelope protein; antigen retrieval may be required.
- ELISA detection may be used for native or recombinant Emerin; consider coating buffer and blocking conditions to maintain protein conformation.
CamelBio: Your One-Stop Sourcing Bridge
CamelBio provides diagnostic manufacturers and researchers with a streamlined one-stop sourcing platform for IVD raw materials, from early-stage development to clinical application. This Emerin/EMD antibody supports studies on nuclear envelope dynamics and Emery-Dreifuss muscular dystrophy, and can be complemented by our validated antibody pairs, optimized monoclonal/polyclonal reagents, and ancillary raw-material supply services. For rare-target or custom sourcing needs, our team bridges the gap with efficient procurement and technical consultation.
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