Anti-ERC1 Rabbit Polyclonal Antibody for WB, ELISA - Q8IUD2

Core Product Specifications and Parameters

Biological Background: ERC1 Function and Localization

• ERC1, also known as ELKS/Rab6-interacting/CAST family member 1 or Rab6-interacting protein 2, is a regulatory subunit of the IKK complex that likely recruits NFKBIA/IkappaBalpha to the complex.
• It plays a key role in the organization of the cytomatrix at the active zone (CAZ) of nerve terminals, which is critical for neurotransmitter release.
• ERC1 may also be involved in vesicle trafficking at the CAZ and in Rab-6-regulated transport from endosomes to the Golgi apparatus.
• The protein is widely expressed, with isoform 2 and isoform 4 abundantly expressed in the brain, while isoform 1 and isoform 3 are predominantly expressed in testis and thyroid.
• Subcellularly, ERC1 localizes to the cytoplasm, cytoskeleton, centrosome, Golgi apparatus membrane, presynaptic cell membrane, and cell projections such as podosomes.
• Key post-translational modifications include acetylation and phosphorylation, and the protein contains coiled-coil domains, consistent with roles in protein transport and membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 700-850 of human ERC1, a region likely selected for specificity across isoforms; consider verifying target identity using knockout or siRNA controls in relevant cell lines.
• For Western blot applications, the predicted protein weight is approximately 128 kDa; use an appropriate gel percentage (e.g., 6-10% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• Validate cross-reactivity with mouse and rat samples, as listed, but confirm performance in your specific experimental context.
• For ELISA, plate coating and antibody dilution should be optimized; a checkerboard titration is recommended to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-ERC1 reagent, and bulk ancillary reagents. Whether you are developing assays for NF-κB signaling, synaptic function, or membrane trafficking, CamelBio offers comprehensive sourcing support from concept to clinic. Our portfolio ensures reliable supply for rare and specialized targets, enabling your diagnostic and research workflows.