Anti-ERK5 Rabbit Monoclonal Antibody for WB, ELISA - Q13164

Core Product Specifications and Parameters

Biological Background: ERK5 Function and Localization

• ERK5, also known as MAPK7, BMK-1, or ERK-5, is a serine/threonine-protein kinase belonging to the MAP kinase family. It plays a central role in the MAPK/ERK signaling cascade, transmitting extracellular signals to regulate diverse cellular processes including proliferation, differentiation, and survival.
• Upon activation by its upstream kinase MAP2K5, ERK5 translocates to the nucleus and phosphorylates downstream targets such as MEF2C. EGF activates ERK5 through a Ras-independent, MAP2K5-dependent pathway, distinguishing it from the classical ERK1/2 modules.
• In cardiomyocytes, ERK5 acts as a negative regulator of apoptosis by interacting with STUB1/CHIP, promoting STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM, thereby contributing to cardioprotective signaling.
• ERK5 may be involved in muscle cell differentiation and is important for endothelial function and the maintenance of blood vessel integrity, highlighting its roles in cardiovascular and muscle biology.
• ERK5 phosphorylates SGK1 at Ser-78, a modification required for growth factor-induced cell cycle progression. Additionally, it participates in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
• The protein localizes to the cytoplasm, nucleus, and PML nuclear bodies, consistent with its nucleocytoplasmic shuttling upon activation and its involvement in transcriptional regulation.
• Post-translational modifications include acetylation and phosphorylation, which are critical for its kinase activity and signaling functions. ERK5 is expressed in many adult tissues, with high abundance in heart, placenta, lung, kidney, and skeletal muscle, but is not detectable in liver.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic peptide corresponding to amino acids 717–816 of human ERK5. It is designed to detect full-length ERK5; however, users should verify its reactivity with specific isoforms or cleavage products in their experimental systems.
• The antibody is validated for western blot (WB) and ELISA applications. For WB, consider using appropriate loading controls and verifying the expected band at ~88 kDa. Cross-reactivity with mouse and rat samples has been reported, but users should confirm performance in their specific tissue or cell line models.
• As ERK5 signaling is influenced by various growth factors and stimuli, ensure that sample preparation and stimulation conditions are optimized to capture the activation state of interest (e.g., phosphorylation-dependent translocation).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-ERK5 antibody exemplifies our capability to supply validated monoclonal antibodies for MAPK signaling research and assay development. Beyond individual antibodies, CamelBio provides optimized antibody pairs, bulk ancillary reagents, and assistance in sourcing rare-target raw materials, ensuring seamless procurement to accelerate your projects.