Anti-ERCC1 Rabbit Polyclonal Antibody for WB and ELISA - P07992

Core Product Specifications and Parameters

Biological Background: ERCC1 Function and Localization

• DNA excision repair protein ERCC-1 (ERCC1), also known as UV20, COFS4, and RAD10, is a non-catalytic component of a structure-specific DNA repair endonuclease.
• It is responsible for the 5'-incision during DNA repair and plays a critical role in the repair of interstrand cross-links (ICL) in conjunction with SLX4.
• ERCC1 participates in processing anaphase bridge-generating DNA structures, which arise from incompletely processed DNA lesions during S or G2 phase and can lead to cytokinesis failure if unresolved.
• The protein is also required for homology-directed repair (HDR) of DNA double-strand breaks, working with SLX4.
• Notably, ERCC1 is not functional in the nucleotide excision repair (NER) pathway, indicating a specialized role in other repair mechanisms.
• It localizes primarily to the nucleus but can also be found in the cytoplasm.
• Post-translational modifications include acetylation and ubiquitination (isopeptide bond formation), and the protein undergoes alternative splicing.
• The molecular weight of the canonical isoform is approximately 33 kDa, consistent with its role in DNA damage response and repair.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to the full-length human ERCC1 (aa 1-323). As a polyclonal antibody, it may recognize multiple epitopes, offering robust detection in Western blot (WB) across human, mouse, and rat lysates.
• For ELISA, consider using the antibody as a capture or detection reagent, but validate pairing and dilutions empirically in your specific assay system.
• Since ERCC1 is involved in DNA repair, ensure your sample preparation preserves nuclear and cytoplasmic fractions if studying localization or post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-ERCC1 rabbit polyclonal antibody supports research into DNA damage, repair mechanisms, and genomic stability. As your sourcing partner, we offer a growing portfolio of validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored rare-target raw-material sourcing to streamline your assay development.