Anti-CXCL1 Rabbit Monoclonal Antibody for WB, ELISA - P12850

Core Product Specifications and Parameters

Biological Background: CXCL1 Function and Localization

• CXCL1, also known as growth-regulated alpha protein, KC, or N51, is a member of the C-X-C motif chemokine family in mouse.
• It functions as a potent chemoattractant for neutrophils, contributing to neutrophil activation during inflammation (By similarity).
• In addition to its chemotactic role, CXCL1 acts as a hematoregulatory chemokine; it suppresses hematopoietic progenitor cell proliferation in vitro, and its truncated form KC(5-72) shows enhanced hematopoietic activity.
• The protein is secreted and contains conserved disulfide bonds typical of chemokines.
• Key post-translational modifications and structural features include signal peptide cleavage and disulfide bond formation; the mature protein has a molecular weight of approximately 10 kDa (10,254 Da based on sequence).
• CXCL1 is annotated with keywords: 3D-structure, Cytokine, Disulfide bond, Growth factor, Inflammatory response, Secreted, Signal.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using a high-percentage SDS-PAGE gel (e.g., 15%) and a low-molecular-weight ladder to resolve the 10 kDa band.
• For ELISA applications, the antibody may serve as a capture or detection reagent; validate its performance in the relevant sample matrix using recombinant mouse CXCL1 as a standard.
• Since the immunogen spans amino acids 25–96 of the mature protein, the antibody likely recognizes the secreted form; confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, such as this Abclonal anti-CXCL1 monoclonal antibody, supporting inflammation and chemokine signaling research. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, helping you streamline assay development from concept to clinical application.