Anti-CD117/c-Kit Rabbit Monoclonal Antibody for WB, ELISA - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• KIT encodes the mast/stem cell growth factor receptor Kit, a transmembrane tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF. It is also known as proto-oncogene c-Kit, p145 c-kit, and CD117.
• Upon ligand binding, KIT activates multiple signaling pathways including PI3K/AKT1, RAS/RAF/MAPK, and phospholipase C gamma (PLCG1), leading to the production of second messengers DAG and IP3. It phosphorylates substrates like PIK3R1, PLCG1, CBL, SRC, and SHC1, and promotes STAT transcription factor activation.
• KIT is essential for a wide range of biological processes: cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and mast cell development, migration, and function.
• Subcellularly, the receptor is located primarily in the cell membrane but can also be found in the cytoplasm. Tissue-specific expression includes spermatogonia and Leydig cells in the testis, and it is maintained in ejaculated spermatozoa. Related references: PMID:20601678
• The protein undergoes post-translational modifications such as glycosylation, disulfide bond formation, and phosphorylation (tyrosine kinase activity), and is subject to ubiquitination, internalization, and degradation upon activation.
• Alternative splicing yields multiple isoforms; disease variants are associated with piebaldism and gastrointestinal stromal tumors. It belongs to the immunoglobulin domain-containing receptor tyrosine kinase family.

Experimental Guidance and Technical Tips

• This antibody, raised against the extracellular domain (aa 26-520), is suitable for Western blotting (WB) and ELISA. In WB, a band at approximately 110 kDa is expected under reducing conditions.
• Cross-reactivity is confirmed for human samples; the suitability for other species should be experimentally determined.
• For ELISA applications, consider using the antibody as a capture or detection reagent, and validate performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop sourcing partner for IVD raw materials, CamelBio offers high-quality antibodies like this anti-CD117/c-Kit rabbit monoclonal antibody to support research in oncology, hematopoiesis, and stem cell biology. Customers can also access a wide range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline diagnostic development from concept to clinic.