Anti-Claudin-4 Rabbit Monoclonal Antibody for WB, IP, ELISA - O14493

Core Product Specifications and Parameters

Biological Background: Claudin-4 Function and Localization

• Claudin-4, encoded by the CLDN4 gene, is a member of the claudin family of tight junction proteins and also functions as the Clostridium perfringens enterotoxin receptor (CPE-R).
• It localizes to tight junctions at cell–cell contacts and is embedded in the cell membrane, consistent with its role in paracellular barrier and ion transport.
• Claudin-4 associates with other claudins to regulate tight junction strand dynamics, influencing both barrier function and ion selectivity. Related references: PMID:35773259 PMID:36008380
• It may coassemble with CLDN8 to form anion-selective channels mediating paracellular chloride permeability in renal collecting ducts. Related references: PMID:36008380
• Integration of claudin-4 into CLDN3 strands can modulate localized tight junction barrier properties. Related references: PMID:35773259 PMID:36008380
• Claudin-4 can interfere with the assembly of cation-selective claudin-2 or claudin-15 strands, inhibiting cation conductance. Related references: PMID:35773259 PMID:36008380
• Remarkably, claudin-4 cannot form tight junction strands independently, highlighting its role as a modulator rather than a pore-former. Related references: PMID:35773259 PMID:36008380
• Post-translational modifications such as phosphorylation and disulfide bond formation are predicted, and the protein is implicated in the Williams-Beuren syndrome chromosomal region.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide encompassing amino acids 111–209 of human claudin-4 (NP_001296.1). Based on the immunogen design, it may detect total claudin-4 protein in denaturing and native conditions, making it suitable for Western blot (predicted ~22 kDa), immunoprecipitation, and ELISA.
• For Western blot, consider using whole-cell lysates from human epithelial or carcinoma cell lines known to express claudin-4, and validate the band size.
• In IP, claudin-4 is a transmembrane protein with extracellular loops; use suitable lysis buffers to solubilize tight junction complexes, and verify co-immunoprecipitated interactors like claudin-3 or claudin-8.
• Given its role as a CPE receptor, the antibody may be useful in functional studies examining Clostridium perfringens enterotoxin binding or internalization.
• As performance may vary, we recommend empirical optimization of working dilutions and experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development with a comprehensive range of high-quality raw materials. This anti-claudin-4 antibody expands our portfolio of tight junction and epithelial cell target reagents, supporting your research from assay conception to clinical application. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—bridging the gap between your project and reliable supply.