Anti-CXCL10/IP-10 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P02778

Core Product Specifications and Parameters

Biological Background: CXCL10/IP-10 Function and Localization

• CXCL10 (IP-10) is a pro-inflammatory C-X-C motif chemokine that regulates chemotaxis, differentiation, and activation of immune cells, as well as cell growth, apoptosis, and angiostatic effects (PubMed:11157474, PubMed:22652417, PubMed:7540647). Related references: PMID:11157474 PMID:22652417 PMID:7540647
• It plays a critical role during viral infections by stimulating immune cell activation and migration to infected sites (By similarity).
• CXCL10 binding to its receptor CXCR3 activates G protein-mediated signaling, leading to phospholipase C activation, intracellular calcium increase, and actin reorganization (PubMed:12750173, PubMed:19151743). Related references: PMID:12750173 PMID:19151743
• The CXCL10/CXCR3 axis recruits activated Th1 lymphocytes to sites of inflammation, a key event in adaptive immune responses (PubMed:12663757, PubMed:12750173). Related references: PMID:12663757 PMID:12750173
• In the central nervous system, CXCL10 promotes microglia activation and migration to brain injury lesions, facilitating neuronal reorganization (By similarity).
• CXCL10 is predominantly secreted by monocytes, endothelial cells, and fibroblasts, and is expressed in thymic epithelial cells (PubMed:11157474) and microglial cells upon viral stimulation (PubMed:12663757). Related references: PMID:11157474 PMID:12663757
• The mature protein is a secreted, disulfide-linked cytokine with a predicted molecular weight of 10.9 kDa, and it undergoes post-translational modifications such as citrullination.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 22-98 of human CXCL10, corresponding to the mature secreted form, suggesting specific detection of the processed chemokine.
• For Western blotting, a band around 11 kDa is expected; include appropriate positive controls (e.g., stimulated monocyte or endothelial cell lysates) to confirm band identity.
• In immunohistochemistry and immunofluorescence, optimize antibody dilution and consider antigen retrieval conditions for paraffin-embedded tissue sections.
• This rabbit monoclonal antibody is suitable for ELISA applications; pairing with a validated capture antibody may enable quantitative detection of circulating CXCL10.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a streamlined supply of IVD raw materials, including high-quality monoclonal antibodies like this anti-CXCL10 clone for inflammation and immunology assays. Our platform offers validated antibody pairs, bulk ancillary reagents, and expert sourcing of rare targets to accelerate your project from concept to clinical application. Partner with CamelBio for the reliable reagents and technical support your assay development demands.