Anti-Cdx1 Rabbit Monoclonal Antibody for WB, ELISA - P47902

Core Product Specifications and Parameters

Biological Background: Cdx1 Function and Localization

• Homeobox protein CDX-1 (Caudal-type homeobox protein 1) is a transcription factor that plays a role in developmental processes and transcriptional regulation (PubMed:24623306).
  Related references: PMID:24623306
• In colorectal cancer cells, CDX-1 is involved in activated KRAS-mediated transcriptional activation of PRKD1 by binding to the PRKD1 promoter (PubMed:24623306).
  Related references: PMID:24623306
• CDX-1 may contribute to the terminal differentiation of the intestinal epithelium, consistent with its tissue-specific expression.
• CDX-1 binds preferentially to methylated DNA, suggesting a role in epigenetic regulation (PubMed:28473536).
  Related references: PMID:28473536
• Subcellular localization is primarily nuclear, consistent with its function as a DNA-binding transcription factor.
• The protein contains a homeobox domain and is classified under keywords such as DNA-binding, nucleus, activator, and developmental protein.
• Alternative splicing generates multiple isoforms, and the protein has been identified through proteomics as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human Cdx1. For Western blot (WB), consider using whole-cell lysates from human colorectal cancer cell lines (e.g., HCT116, SW480) or intestinal tissue samples to detect the expected 28kDa band.
• For ELISA, the synthetic peptide immunogen can be employed as a positive control antigen; validate the assay conditions with a known Cdx1-expressing sample.
• Cross-reactivity has only been confirmed for human. If using samples from other species, verify sequence homology of the immunogen region and perform a positive control validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined portal for IVD raw-material procurement. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your Cdx1-related research in colorectal cancer and intestinal biology. For rare or custom targets, our sourcing services bridge the gap from concept to clinic, ensuring you find the right raw materials for your assay development.