Anti-CCR8 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P51685

Core Product Specifications and Parameters

Biological Background: CCR8 Function and Localization

• CCR8 is a G protein-coupled receptor belonging to the chemokine receptor family. It exists in alternatively spliced isoforms and contains structural hallmarks including seven transmembrane helices and disulfide bonds.
• It functions as a receptor for chemokines CCL1, CCL8, CCL16, and CCL18, regulating monocyte and eosinophil chemotaxis. CCL18 binding induces receptor internalization and migration of highly polarized Th2 cells; in microglial cells, CCL18 promotes phagocytosis through NF-kappa-B and Src signaling pathways. Related references: PMID:23999500 PMID:35041514
• The CCL1-CCR8 signaling axis protects the gut from acute intestinal damage.
• CCR8 acts as a coreceptor for HIV-1 and HIV-2 viruses, facilitating viral entry.
• It also serves as a receptor for the Kaposi sarcoma-associated herpesvirus protein vCCL1/K6, which inhibits apoptosis in targeted cells.
• Localized at the cell membrane, CCR8 is a multi-pass membrane protein.
• It is expressed in unstimulated CD4+ and CD8+ T-cells and polymorphonuclear cells, highlighting its role in immune cell trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the C-terminal intracellular region (amino acids 250-355). For Western blotting, denaturing conditions ensure accessibility; a band near 41 kDa is expected.
• For immunofluorescence/ICC, permeabilization may be required to allow antibody access to the intracellular epitope. Validate the antibody in relevant human or mouse cell models.
• In ELISA, consider using the peptide as a positive control and titrate the antibody for optimal signal-to-noise ratio.
• Given the cross-reactivity with mouse, the antibody may recognize conserved sequences; verify performance if using mouse tissues or cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for immunoassay development. For CCR8-related projects in immunology and inflammation, we can source rare-target raw materials and deliver them efficiently from concept to clinic. Whether you need custom antibody generation or reliable supply of critical components, our team is ready to bridge your procurement needs.