Anti-CXCR3 Rabbit Monoclonal Antibody for WB and ELISA - P49682

Core Product Specifications and Parameters

Biological Background: CXCR3 Function and Localization

• CXCR3, also known as GPR9 or CD183, is a G protein-coupled receptor encoded by the CXCR3 gene. Alternative splicing produces at least two isoforms with distinct tissue expression profiles.
• It functions as a receptor for the C-X-C chemokines CXCL9, CXCL10, and CXCL11, mediating proliferation, survival, and angiogenic activity in human mesangial cells through heterotrimeric G-protein signaling, and promoting chemotaxis via Ras/ERK pathway activation. Related references: PMID:12782716
• CXCR3 also binds CXCL4, mediating anti-proliferative and anti-angiogenic effects in human microvascular endothelial cells through a cAMP-dependent pathway and p38MAPK activation. Overexpression in renal cancer cells downregulates HMOX1 and promotes apoptosis. Related references: PMID:12782716
• The receptor mediates the specific activity of CXCL11.
• Subcellularly, CXCR3 is localized to the cell membrane.
• Isoforms 1 and 2 are predominantly expressed in heart, kidney, liver, and skeletal muscle; isoform 1 is also found in placenta, while isoform 2 is expressed in endothelial cells. At the protein level, CXCR3 is detected in T-cells.
• Post-translational modifications include glycosylation, sulfation, and disulfide bond formation, consistent with its classification as a glycoprotein and transmembrane receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular region (amino acids 200–300); for Western blot, consider using reducing conditions to ensure complete denaturation.
• Validated applications are WB and ELISA; performance in other assays (e.g., IP, IF, IHC) may require empirical optimization.
• Cross-reactivity is reported for human; when using with other species, verify sequence homology to avoid non-specific binding.
• Expect a band near 41 kDa in WB; post-translational modifications like glycosylation may cause slight mobility shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with a comprehensive portfolio that includes validated CXCR3 antibodies, antibody pairs, and ancillary reagents. Whether your project requires bulk quantities, custom formulations, or rare-target sourcing for chemokine receptor research, our one-stop supply chain supports your diagnostic development from concept to clinic. Partner with us to accelerate your immunology and oncology assay programs.