Anti-TCL1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P56279

Core Product Specifications and Parameters

Biological Background: TCL1 Function and Localization

• TCL1A, also known as T-cell leukemia/lymphoma protein 1A or oncogene TCL1, is a proto-oncogene implicated in T-cell malignancies through chromosomal rearrangements.
• It functions as an AKT co-activator, enhancing the phosphorylation and activation of AKT1, AKT2, and AKT3, thereby promoting cell proliferation and survival.
• TCL1A promotes the nuclear translocation of AKT1, contributing to its signaling roles in the nucleus.
• It stabilizes mitochondrial membrane potential, further supporting cell survival under stress conditions.
• The protein is primarily localized in the cytoplasm and nucleus, and also found in microsomal and endoplasmic reticulum fractions.
• TCL1A is specifically expressed in immature thymocytes and activated peripheral lymphocytes, highlighting its role in early T- and B-lymphocyte differentiation.
• No direct post-translational modifications are annotated, but it is identified in proteomics studies.

Experimental Guidance and Technical Tips

• For WB, consider using whole cell lysates from human T-cell lines or activated lymphocytes, as TCL1 expression is restricted to these cell types.
• In IF/ICC, validate the antibody under permeabilized conditions to detect both cytoplasmic and nuclear localization.
• For ELISA, use recombinant human TCL1 protein as a standard for quantification and assay optimization.
• Due to the immunogen covering amino acids 1-114, the antibody recognizes the N-terminal region, which may be suitable for detecting full-length and truncated forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop platform for IVD raw material sourcing, including validated antibody pairs and optimized monoclonal antibodies like this anti-TCL1 clone. We support diagnostic manufacturers with bulk ancillary reagents, rare target raw-material procurement, and customized production services. Our expertise spans the entire development pathway, helping you accelerate your concept-to-clinic journey with reliable supply solutions.