Anti-CXCR5/CD185 Rabbit Monoclonal Antibody for WB, FC, ELISA - P32302

Core Product Specifications and Parameters

Biological Background: CXCR5 Function and Localization

• CXCR5 (C-X-C chemokine receptor type 5), also known as Burkitt lymphoma receptor 1 (BLR1) or monocyte-derived receptor 15 (MDR-15), is a G protein-coupled receptor (GPCR) that functions as a cytokine receptor.
• It specifically binds the B-lymphocyte chemoattractant (BLC), mediating B-cell migration into B-cell follicles of the spleen and Peyer patches, essential for humoral immunity.
• CXCR5 plays a potential regulatory role in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation, as indicated by its expression in BL cells.
• The receptor is localized at the cell membrane and contains seven transmembrane helices characteristic of GPCRs.
• Post-translational modifications include disulfide bond formation and glycosylation, which are important for receptor folding and function.
• Tissue expression is primarily in mature B-cells and Burkitt lymphoma cells, consistent with its role in B-cell biology.
• Alternative splicing has been observed for this gene, potentially generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–47 of human CXCR5, encompassing the N-terminal extracellular domain. This design is suitable for applications like flow cytometry (FC) and ELISA, where surface-exposed epitopes are accessible.
• For Western blot (WB), the predicted molecular weight is approximately 42 kDa; however, glycosylation may cause a higher apparent molecular weight. Consider using appropriate deglycosylation controls if needed.
• Validate the antibody's specificity in human B-cell lines (e.g., Raji, Daudi) or Burkitt lymphoma cells, and test for potential cross-reactivity with other chemokine receptors if pan-specificity is a concern.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams by providing a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-CXCR5/CD185 antibody. Whether you need bulk ancillary reagents or rare-target sourcing for B-cell-related disease diagnostics, our one-stop procurement bridge streamlines your development from concept to clinic.