Anti-CXCL9/MIG Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q07325

Core Product Specifications and Parameters

Biological Background: CXCL9 Function and Localization

• C-X-C motif chemokine 9 (CXCL9), also known as Monokine induced by interferon-gamma (MIG), is a small cytokine belonging to the CXC chemokine family.
• Encoded by the CXCL9 gene, the protein is synthesized as a precursor with a signal peptide, processed into a mature ~14 kDa secreted form.
• CXCL9 is a potent chemoattractant for activated T-cells, directing their migration to sites of inflammation through specific binding to the CXCR3 receptor.
• It is secreted by various cell types, including macrophages and endothelial cells, primarily in response to interferon-gamma (IFN-γ) stimulation.
• The mature chemokine contains conserved disulfide bonds that are critical for its three-dimensional structure and biological activity.
• As a pro-inflammatory cytokine, CXCL9 plays a central role in Th1-type immune responses and is implicated in the pathogenesis of autoimmune disorders and tumor immune surveillance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–125, representing the mature, secreted form of CXCL9, suggesting the antibody likely recognizes the cleaved, active chemokine.
• For Western blot (WB), the predicted molecular weight is ~14 kDa; consider using a high-percentage gel (e.g., 15% SDS-PAGE) and optimize transfer conditions for low-molecular-weight proteins.
• In immunofluorescence (IF/ICC), detection of secreted CXCL9 may require permeabilization and careful fixation to retain the extracellularly bound or matrix-associated protein.
• For ELISA applications, pairing this monoclonal antibody with a detection antibody that targets a distinct epitope could enhance assay specificity and sensitivity.
• Cross-reactivity is confirmed for human CXCL9; when working with other species, validate in the relevant sample system before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material partner, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research labs. This CXCL9/MIG monoclonal antibody supports immunology and inflammation research, and we can assist with rare target raw-material sourcing, technical consulting, and tailored supply solutions to accelerate your project from concept to clinic.