Anti-CCL4/MIP-1 beta Monoclonal Antibody for WB, IF/ICC, ELISA - P14097

Core Product Specifications and Parameters

Biological Background: CCL4/MIP-1 beta Function and Localization

• C-C motif chemokine 4 (CCL4), also known as macrophage inflammatory protein 1-beta (MIP-1-beta) or ACT-2, is a member of the CC chemokine family, encoded by the Ccl4 gene in murine species.
• The protein functions as a cytokine with inflammatory and chemokinetic properties, it is involved in the recruitment of immune cells to sites of inflammation and infection.
• CCL4 is a secreted signaling molecule that mediates its effects through interactions with specific chemokine receptors, contributing to the regulation of immune cell trafficking and activation.
• According to UniProt annotation, the protein contains a disulfide bond, a common structural feature of chemokines critical for maintaining their three-dimensional conformation and biological activity.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 24-92 of mouse CCL4) corresponds to the mature secreted peptide region; the antibody thus may recognize the processed, active form of the protein.
• For Western blotting (WB), consider using reducing sample preparation to account for potential disulfide bonding, and verify detection in relevant biological samples such as culture supernatants or tissue homogenates.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization may be required to detect intracellular CCL4, although secreted protein may also be visualized on cell surfaces; appropriate controls are recommended.
• Cross-reactivity with human and rat species, as reported, suggests that the epitope is conserved; experimental validation in the target species and matrix is always advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying diagnostic manufacturers and laboratories with comprehensive IVD raw material solutions from concept to clinic. For CCL4/MIP-1 beta research and immunoassay development, we offer validated antibody pairs, recombinant proteins, and ancillary reagents for bulk procurement. Our sourcing capabilities extend to rare targets, ensuring you have the critical materials for inflammation and chemokine-related projects.