CXCR7 Rabbit mAb for WB, ELISA - P25106

Core Product Specifications and Parameters

Biological Background: CXCR7 Function and Localization

• CXCR7 (ACKR3) is an atypical chemokine receptor that binds the chemokines CXCL11 and CXCL12/SDF1 with high affinity, but does not activate classic G protein-mediated signal transduction. Related references: PMID:16107333 PMID:19255243 PMID:19380869
• Instead, ligand binding induces beta-arrestin recruitment, leading to chemokine internalization, degradation or transcytosis, and activation of the MAPK signaling pathway, thereby scavenging and regulating chemokine levels. Related references: PMID:16940167 PMID:18653785 PMID:20018651
• Plays a regulatory role in neuronal migration by modulating CXCR4 protein levels in migrating interneurons, and is required for heart valve development and oculomotor axon guidance. Related references: PMID:16940167 PMID:18653785 PMID:17804806 PMID:31211835
• In glioma and malignant hematopoietic cells, CXCR7 transduces signals via the MEK/ERK pathway, promoting cell growth, survival, adhesion, and migration. Related references: PMID:16940167 PMID:20388803 PMID:17804806 PMID:18653785 PMID:19641136
• Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates during microbial infection.
• Localizes primarily to the cell membrane and cycles through early and recycling endosomes.
• Expressed in monocytes, B-cells, endothelial cells, and various neuronal and kidney cell types; highly upregulated in astroglial tumors and several myeloid malignancies but low in normal progenitors.
• Key post-translational modifications include glycosylation and phosphorylation; the protein is a seven-transmembrane domain receptor with a molecular weight of ~41.5 kDa, detected by this antibody at ~41 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular region (aa 263-362) of human CXCR7, making it suitable for detecting denatured protein in WB and ELISA formats.
• For Western blot, using reducing and denaturing conditions is recommended; the expected band is ~41 kDa. Validate signal in a positive control such as human glioblastoma or leukemia cell lysates.
• In ELISA, the antibody can be used for direct antigen detection; consider immobilizing recombinant CXCR7 protein or cell membrane extracts to assess binding.
• Cross-reactivity has been confirmed only for human CXCR7; for other species, check sequence alignment of the immunogen region before use.
• Although not listed, the antibody may be tested in IHC/IF with proper antigen retrieval, given that the epitope is intracellular; however, empirical optimization is required.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing partner for IVD raw materials, CamelBio supports diagnostic manufacturers and research laboratories by providing high-quality reagents like this CXCR7 rabbit monoclonal antibody. Our supply network covers validated antibody pairs, optimized recombinant proteins, and ancillary reagents for chemokine receptor research. Whether you need bulk quantities of this anti-CXCR7 antibody or are searching for rare-target raw materials, CamelBio streamlines procurement from concept to clinic.