Anti-CCR6/CD196 Rabbit Monoclonal Antibody (FC, ELISA) - O54689

Core Product Specifications and Parameters

Biological Background: CCR6/CD196 Function and Localization

• C-C chemokine receptor type 6 (CCR6), also known as KY411, is a G protein-coupled receptor that specifically binds the chemokine CCL20, triggering intracellular calcium mobilization and signal transduction. Related references: PMID:20068036
• CCR6 also functions as a receptor for beta-defensins such as DEFB1, DEFB4, and DEFB4A/B, mediating chemotaxis and bactericidal activity; this non-chemokine ligand interaction expands its role in innate immunity. Related references: PMID:20068036 PMID:25122636
• The receptor is critical for immune cell trafficking: it directs the chemotaxis of dendritic cells, effector/memory T cells, B cells, and Th17/Treg subsets to skin and mucosal sites, balancing inflammatory and regulatory responses. Related references: PMID:19050256 PMID:19129757
• In the intestinal mucosa, CCR6-mediated signals orchestrate immune responses against microbes and modulate tissue inflammation; it is required for normal Th17 cell migration in Peyer’s patches and lamina propria. Related references: PMID:21376174 PMID:19129757
• Beyond immune cells, CCR6 is expressed in sperm, where it positively regulates motility and chemotaxis via CCL20, and is localized to the principal piece, neck, and acrosomal regions. Related references: PMID:23765988
• Subcellularly, CCR6 is a multi-pass membrane protein found on the cell surface; it contains disulfide bonds and is glycosylated, typical of chemokine receptors.
• Tissue expression is prominent in testis, lung, colon, and dendritic cells, with protein-level presence in various T helper and regulatory T cell subsets, memory B cells, and thymocyte precursors.
• Plays a role in B-cell biology: essential for B-cell localization in the subepithelial dome of Peyer’s patches, efficient IgA class switching, and memory B-cell distribution in the spleen for secondary recall responses. Related references: PMID:27174992 PMID:25505290

Experimental Guidance and Technical Tips

• The immunogen (N-terminal extracellular domain, aa 2-38) is involved in ligand binding; consider testing the antibody in neutralization or receptor-blocking functional assays if applicable.
• For flow cytometry, employ standard surface staining protocols on live cells; validate detection of endogenous CCR6 in relevant mouse samples (e.g., splenocytes, thymocytes, or isolated T-cell subsets).
• In ELISA, the antibody may serve as either capture or detection reagent; empirical optimization of dilution and pairing with appropriate standards is advised.
• Reported reactivity is for mouse; cross-reactivity with human or rat has not been tested—verify if using beyond mouse.
• The predicted molecular weight is 42 kDa; if adapting the antibody for Western blot, confirm band specificity with proper controls, noting the product is currently validated only for FC and ELISA.
• Shipping is on ice bags; store appropriately and avoid repeated freeze-thaw cycles to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated B2B partner for IVD raw materials, providing one-stop access to high-quality antibodies like this CCR6/CD196 rabbit mAb. Whether you need validated antibody pairs for immunoassay development, optimized monoclonal antibodies for flow or ELISA, or large-scale ancillary reagents, we bridge the gap from concept to clinic. This anti-CCR6 antibody serves immunology and inflammation research, supporting studies on chemokine signaling, T-cell trafficking, and mucosal immunity.