Anti-CXCR5 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P32302

Core Product Specifications and Parameters

Biological Background: CXCR5 Function and Localization

• C-X-C chemokine receptor type 5 (CXCR5), also known as Burkitt lymphoma receptor 1 (BLR1) and monocyte-derived receptor 15 (MDR-15), is a G protein-coupled receptor (GPCR) encoded by the CXCR5 gene (Gene ID: 643) in humans.
• CXCR5 functions as a specific receptor for B-lymphocyte chemoattractant (BLC/CXCL13) and mediates B-cell homing and migration into B-cell follicles of spleen and Peyer’s patches, but not mesenteric or peripheral lymph nodes.
• It may play a regulatory role in Burkitt lymphoma lymphomagenesis and/or B-cell differentiation, consistent with its expression in mature B-cells and Burkitt lymphoma cells.
• As a typical GPCR, CXCR5 is a multi-pass membrane protein localized at the cell membrane, featuring transmembrane helices, disulfide bonds, and N-linked glycosylation.
• The receptor is involved in B-cell activation and chemotactic signal transduction, linking chemokine binding to intracellular G protein-mediated pathways.
• Alternative splicing of the CXCR5 transcript may generate isoforms with distinct functional properties, though specific variants are not detailed in the current record.
• Keywords associated with CXCR5 include B-cell activation, G protein-coupled receptor, glycoprotein, disulfide bond, and transmembrane, reflecting its role as a cell surface receptor involved in adaptive immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 273-372 of human CXCR5, a region likely exposed on the cytoplasmic side or in an extracellular loop; users should consider this when interpreting signal in full-length vs. truncated forms.
• For Western blot (WB), CXCR5 has a predicted molecular weight of 42 kDa; ensure gel percentage and transfer conditions are optimized for multi-pass membrane proteins, which may require extended transfer times or special buffers.
• Detection in immunofluorescence (IF) protocols may benefit from gentle permeabilization to allow antibody access to the intracellular epitope region; validate staining in known CXCR5-positive cell types such as mature B cells.
• ELISA applications should include appropriate controls, as the antibody may recognize denatured or linear epitopes presented by the synthetic peptide, which could affect binding to native folded receptor.
• Cross-reactivity with mouse and rat CXCR5 is reported; however, always verify reactivity and specificity in your species and tissue of interest, particularly for applications beyond those listed.
• Membrane proteins like CXCR5 can be sensitive to sample preparation; avoid high heat and use reducing agents cautiously to prevent aggregation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this high-quality anti-CXCR5 rabbit monoclonal antibody suitable for B-cell immunology and lymphoma studies. Beyond antibodies, we offer access to validated antibody pairs, bulk recombinant proteins, and a wide range of ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing expertise bridges the gap from concept to clinic.