Anti-CX3CL1 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - O35188

Core Product Specifications and Parameters

Biological Background: CX3CL1 Function and Localization

• Fractalkine (CX3CL1), also known as ABCD-3 or Neurotactin, is the sole member of the CX3C chemokine subfamily and exists in both membrane-anchored and proteolytically shed soluble forms.
• Fractalkine binds with high affinity to the CX3CR1 receptor and the integrins ITGAV:ITGB3 and ITGA4:ITGB1, activating downstream signaling that regulates immune cell adhesion and chemotaxis. Related references: PMID:10187784, PMID:18971423
• In the vasculature, the CX3CL1/CX3CR1 axis mediates firm adhesion of leukocytes to endothelial cells under flow, a critical step in immune surveillance and inflammation. Related references: PMID:10382755, PMID:9177350
• The membrane-anchored form promotes rapid capture and adhesion of immune cells under shear flow, while the released soluble chemokine domain acts as a chemoattractant for T cells and monocytes.
• CX3CL1 is predominantly expressed in the brain, with lower mRNA levels detected in kidney, heart, lung, skeletal muscle, and testis, and it is inducible in dendritic cells and activated B cells.
• Subcellular localization is dynamic: the full-length protein resides on the cell membrane as a type I transmembrane protein with an extracellular chemokine domain, while ADAM-mediated cleavage releases the soluble chemokine segment.
• Key functional keywords include chemotaxis, cell adhesion, inflammatory response, and immune cell trafficking, underscoring its role in both homeostatic and pathological immune surveillance.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 25–337, encompassing the entire extracellular domain (chemokine domain and mucin-like stalk) of mouse CX3CL1. This design may enable the antibody to detect both membrane-bound and shed forms.
• For Western blotting, a band around 42 kDa is expected; consider using brain or kidney lysates as positive controls.
• In IHC-P, antigen retrieval optimization and use of CX3CL1-expressing tissues (e.g., brain sections) are recommended.
• Sandwich ELISA development may be feasible by pairing this antibody with a detector antibody targeting a non-overlapping epitope; validate in recombinant protein or native sample contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material procurement, offering a curated portfolio of monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti-CX3CL1 rabbit monoclonal antibody supports immunoassay development in inflammation, neuroimmunology, and cancer research. Let CamelBio accelerate your diagnostic pipeline with reliable supply and expert technical support.