Anti-CHD1 Rabbit Monoclonal Antibody for WB, ELISA - O14646

Core Product Specifications and Parameters

Biological Background: CHD1 Function and Localization

• CHD1 (Chromodomain-helicase-DNA-binding protein 1) is an ATP-dependent chromatin remodeler belonging to the SNF2 family, with alternative splicing generating multiple isoforms.
• Functions as the substrate recognition component of the SAGA complex, regulating RNA polymerase II transcription, and is required for efficient RNA polymerase I transcription termination.
• Required for bridging of SNF2, FACT, PAF, and U2 snRNP complexes to H3K4me3, thereby modulating pre-mRNA splicing efficiency. Related references: PMID:18042460 PMID:28866611
• Negatively regulates DNA replication and maintains specific chromatin configurations across the genome; also associates with histone deacetylase (HDAC) activity.
• Localizes to both the nucleus and cytoplasm, and exhibits tissue-specific expression with highest levels in the brain (cerebellum and basal ganglia).
• Undergoes phosphorylation and is associated with disease variants linked to intellectual disability; required for maintaining open chromatin and pluripotency in embryonic stem cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1510–1710) of human CHD1; this rabbit monoclonal antibody may recognize full-length CHD1 and potentially some isoforms.
• For Western blotting, due to the high molecular weight of CHD1 (~197 kDa), use 6–8% SDS-PAGE gels and optimize transfer conditions (e.g., prolonged transfer time or wet transfer) to ensure efficient detection.
• Validate the antibody in relevant human tissue or cell extracts, particularly those expressing CHD1 such as brain-derived samples.
• For ELISA, the antibody may be employed as a detection reagent; appropriate assay conditions and antibody pairing should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as a one-stop sourcing partner for IVD raw materials, supporting research and manufacturing in chromatin biology and epigenetics. This CHD1 antibody exemplifies our extensive portfolio, which spans validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents to accelerate diagnostic development. Whether you need specific monoclonal antibodies or comprehensive raw-material solutions, CamelBio bridges the gap from concept to clinical application.