Anti-CHDH Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8NE62

Core Product Specifications and Parameters

Biological Background: CHDH Function and Localization

• CHDH (choline dehydrogenase) is a mitochondrial enzyme that catalyzes the oxidation of choline to betaine aldehyde, the first step in the glycine betaine synthesis pathway.
• It is a flavoprotein that uses FAD as a cofactor for its oxidoreductase activity.
• The enzyme is localized to both the inner and outer mitochondrial membranes, indicating dynamic membrane association.
• CHDH is involved in mitochondrial quality control, participating in the initiation of mitophagy after mitochondrial damage. Related references: PMID:25483962
• The human CHDH gene (GeneID: 55349) encodes a protein with a predicted molecular weight of ~65 kDa, which is processed from a precursor containing a mitochondrial transit peptide.
• Post-translational modifications include acetylation, which may regulate enzyme activity or localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 245-594, which encompasses the catalytic domain; the antibody may recognize both native and denatured CHDH in Western blot (WB) analysis.
• For WB, consider using mitochondrial fractions or whole-cell lysates; expect a predominant band near 65 kDa. Due to cross-reactivity with mouse and rat, the antibody can be employed in cross-species studies.
• For IHC-P, optimize antigen retrieval protocols and validate staining in tissues known to express CHDH (e.g., liver, kidney).
• In ELISA, the antibody can serve as a detection reagent; appropriate blocking and dilution steps should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete sourcing solution for IVD raw materials, supplying this anti-CHDH antibody alongside validated antibody pairs, bulk ancillary reagents, and custom rare-target procurement to accelerate your choline metabolism and mitochondrial research. Our integrated supply chain and technical support bridge the gap from concept to clinic, ensuring consistent quality and scalability for diagnostic development.