Anti-CD74 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, FC, ELISA - P04233-2

Core Product Specifications and Parameters

Biological Background: CD74 Function and Localization

• CD74 encodes the HLA class II histocompatibility antigen gamma chain, also known as the invariant chain (Ii) or CLIP (class II-associated invariant chain peptide).
• This isoform (p35) is one of multiple spliced variants, with the canonical isoform serving as a chaperone for MHC class II α/β dimers.
• The invariant chain blocks premature peptide binding in the endoplasmic reticulum and directs MHC class II transport to endosomal compartments.
• Upon proteolytic cleavage, the CLIP fragment remains in the peptide-binding groove until exchanged with antigenic peptides by HLA-DM.
• CD74 is also expressed as a cell surface receptor for macrophage migration inhibitory factor (MIF), implicating it in inflammatory signaling.
• Keywords: Antigen processing and presentation, Chaperone, Immune response, Membrane, Signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 73–232) of CD74, which is conserved across major isoforms, supporting detection of both full-length and processed forms.
• For Western blot, multiple bands (18–34 kDa) may be observed due to alternative splicing and post-translational modifications; validate in relevant human cell lines (e.g., Raji, HeLa).
• In immunohistochemistry or immunofluorescence, antigen retrieval methods and permeabilization steps should be optimized for the target tissue.
• This antibody can be used for flow cytometry to assess surface CD74 expression; include appropriate isotype controls and Fc blocking.
• The recombinant rabbit monoclonal format ensures batch-to-batch consistency, making it suitable for quantitative assays like ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, clinical laboratories, and research institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. This anti-CD74 monoclonal antibody serves as a reliable reagent for immunophenotyping, biomarker studies, and the development of assays targeting MHC class II antigen presentation and immune regulation. For specialized projects, CamelBio also leverages its global network to source rare-target raw materials and provide tailored technical consultation, helping you accelerate from concept to clinic.