Anti-CHD4 Monoclonal Antibody for WB, IHC-P, ELISA - Q14839

Core Product Specifications and Parameters

Biological Background: CHD4 Function and Localization

• CHD4 is a core ATPase subunit of the NuRD (nucleosome remodeling and deacetylase) complex, coupling histone deacetylation to ATP-dependent chromatin remodeling and transcriptional repression. Related references: PMID:16428440 PMID:17626165
• It binds and distorts nucleosomal DNA, facilitating chromatin remodeling during transcription, DNA replication, and repair. Related references: PMID:28977666 PMID:32543371
• Recruited to acetylated damaged chromatin by ZMYND8, CHD4 promotes transcriptional silencing and facilitates double-strand break repair by homologous recombination. Related references: PMID:25593309
• CHD4 is involved in neurogenesis, with functional roles demonstrated in model organisms (By similarity).
• The protein localizes to the nucleus and can also be found in the cytoplasm, associated with the centrosome and microtubule organizing center.
• Widely expressed across tissues, CHD4 is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which regulate its activity and interactions.
• According to UniProt keywords, it is implicated in intellectual disability, contains multiple zinc finger domains, and belongs to the Snf2 family of helicase-related proteins.

Experimental Guidance and Technical Tips

• The immunogen targets the C-terminal region (aa 1520–1700); the antibody may recognize full-length CHD4 and certain isoforms.
• For WB, the predicted molecular weight is ~218 kDa; consider using low-percentage SDS-PAGE gels and prolonged transfers for efficient detection of this large protein.
• For IHC-P, validate antibody specificity with appropriate antigen retrieval methods and isotype controls.
• For ELISA, the recombinant immunogen fragment can serve as a positive control to establish assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. This CHD4 antibody is part of our chromatin/epigenetics range, supported by accessories like validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio streamlines your supply chain with technical consultation and reliable material access.