Anti-CHCHD4 Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - Q8N4Q1

Core Product Specifications and Parameters

Biological Background: CHCHD4 Function and Localization

• CHCHD4, also known as MIA40 or TIMM40, is a mitochondrial intermembrane space import and assembly protein belonging to the coiled-coil-helix-coiled-coil-helix domain-containing family.
• It functions as a central redox-sensitive chaperone, catalyzing disulfide bond formation in substrate proteins (e.g., COA7, COX17, COX19, MICU1, TIMCC/FAM136A) essential for respiratory chain complex biogenesis (PubMed:26004228). Related references: PMID:26004228
• CHCHD4 oxidizes precursor proteins by forming transient intermolecular disulfide bridges, and is then reoxidized by GFER/ERV1 via a disulfide relay system (PubMed:16185709, PubMed:19182799, PubMed:21059946, PubMed:23186364). Related references: PMID:16185709 PMID:19182799 PMID:21059946 PMID:23186364
• It mediates disulfide bond formation in MICU1, promoting MICU1-MICU2 heterodimer formation and thus regulating mitochondrial calcium uptake (PubMed:26387864). Related references: PMID:26387864
• CHCHD4 is localized to the mitochondrial intermembrane space.
• It is ubiquitously expressed across human tissues.
• The mature protein has a calculated molecular mass of approximately 16 kDa (UniProt: 15996 Da).
• Alternative splicing and a redox-active center with disulfide bonds are noted as additional features.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–105) of human CHCHD4; the antibody may detect both precursor and mature forms—validate in your specific sample system.
• For western blotting, expect a primary band near 16 kDa; include reducing agents and appropriate molecular weight markers.
• In immunofluorescence and immunohistochemistry, mitochondrial staining patterns are anticipated; co-stain with organelle markers to confirm localization.
• For paraffin-embedded IHC-P, consider antigen retrieval and signal amplification steps.
• The listed cross-reactivity with mouse and rat suggests utility in rodent models, but verification in the relevant tissue or cell line is recommended.
• ELISA applications may be feasible; optimize coating and detection conditions as per your assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CHCHD4-related projects in mitochondrial biology, oxidative stress, and respiratory chain research, we offer targeted sourcing solutions to accelerate your assay development. Let CamelBio be your one-stop bridge from concept to clinic, providing tailored procurement and technical support for even rare target raw materials.