Anti-CD7 Rabbit Monoclonal Antibody for WB, ELISA - P09564

Core Product Specifications and Parameters

Biological Background: CD7 Function and Localization

• CD7 (also known as GP40, TP41, or Leu-9) is a transmembrane glycoprotein expressed on the surface of T‑cells, natural killer (NK) cells, and their precursors. Related references: PMID:7506726
• Upon binding to its ligand SECTM1/K12, CD7 functions as a costimulatory receptor that enhances T‑cell activation and promotes the secretion of cytokines such as IL‑2. Related references: PMID:10652336, PMID:1709867
• Engagement of CD7 on resting NK cells triggers a robust production of interferon‑gamma. Related references: PMID:7506726
• The protein is anchored in the plasma membrane via a single transmembrane helix, with its extracellular domain containing an immunoglobulin‑like V‑type domain.
• Post‑translational modifications include N‑linked glycosylation and palmitoylation, and the structure is stabilized by conserved disulfide bonds.
• UniProt keywords classify CD7 as involved in adaptive immunity, and it possesses immunoglobulin domain, receptor, and transmembrane helix features.

Experimental Guidance and Technical Tips

• For western blotting, use reducing conditions on whole‑cell lysates from human T‑cell lines (e.g., Jurkat) or NK cells. The expected band appears at approximately 25 kDa; glycosylation may cause a slight upward shift.
• For ELISA, consider using the antibody as a capture or detection reagent; it is advisable to validate its performance in sandwich assays by pairing with other anti‑CD7 clones.
• The immunogen covers residues 26–180 (extracellular domain), suggesting the antibody recognizes an epitope on the native protein; users may wish to evaluate it in other native‑protein applications such as flow cytometry with appropriate validation.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, technical services, and consulting. This anti‑CD7 rabbit monoclonal antibody is ideal for immunology and hematology assay development. As a one‑stop partner, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing materials for rare or emerging targets.