Anti-ACHA4 Rabbit Monoclonal Antibody for WB, ELISA - P43681

Core Product Specifications and Parameters

Biological Background: Neuronal Acetylcholine Receptor Subunit Alpha-4 Function and Localization

• Neuronal acetylcholine receptor subunit alpha-4 (CHRNA4) is a ligand-gated ion channel component that assembles into pentameric cation channels with high calcium permeability, mediating fast excitatory neurotransmission.
• CHRNA4 heteropentamerizes with CHRNB2 or CHRNB4 subunits, and associates with accessory subunits CHRNA5 and CHRNB3, forming the most abundant nAChR subtype in the central nervous system. Related references: PMID:16835356 PMID:22361591 PMID:27698419
• The receptor exists in two major stoichiometric forms, (CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, which differ in unitary conductance, calcium permeability, ACh sensitivity, and divalent cation potentiation. Related references: PMID:27698419 PMID:29720657 PMID:38454578
• Through calcium-dependent signaling, CHRNA4-containing receptors influence the release of neurotransmitters such as dopamine, glutamate, and GABA.
• The protein is localized to the synaptic cell membrane and cell membrane, consistent with its role in synaptic transmission.
• CHRNA4 undergoes alternative splicing and post-translational modifications including glycosylation and palmitoylation; mutations in the gene are associated with epilepsy.
• The mature protein has a molecular weight of approximately 70 kDa (69,957 Da), confirmed by UniProt analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 500–600 of human ACHA4, targeting the C-terminal intracellular region; consider this epitope location when designing detection or blocking experiments.
• For Western blot, ACHA4 is a ~70 kDa protein; using a 10% SDS-PAGE gel and validating with brain tissue lysates where the target is enriched may help confirm specificity.
• In ELISA applications, coating the plate with the immunizing peptide or recombinant ACHA4 protein could serve as a positive control; optimize antibody dilution under your experimental conditions.
• Cross-reactivity with mouse and rat orthologs is reported, but always validate in the relevant sample system, especially if studying species-specific modifications or expression patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consultative support. This ACHA4 rabbit monoclonal antibody belongs to our neuroscience and ion channel portfolio, backed by validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. Whether you need routine antibodies or rare target sourcing, our integrated supply chain bridges your project from concept to clinic.