Anti-Chk2 (CHEK2) Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - O96017

Core Product Specifications and Parameters

Biological Background: Chk2 Function and Localization

• Chk2, encoded by the CHEK2 gene (also known as CDS1, RAD53, HuCds1), is a serine/threonine-protein kinase and tumor suppressor.
• It phosphorylates numerous effectors at the consensus sequence [L-X-R-X-X-S/T], regulating cell cycle arrest, DNA repair, and apoptosis in response to DNA double-strand breaks. Related references: PMID:37943659
• Chk2 inhibits CDC25 phosphatases to block CDK-cyclin activation, phosphorylates p53/TP53 at Ser-20 to stabilize it, and phosphorylates BRCA2 to promote homologous recombination repair.
• It localizes predominantly to the nucleus and PML nuclear bodies, consistent with its role in DNA damage signaling.
• Tissue expression is high in testis, spleen, colon, and peripheral blood leukocytes, with lower levels in other tissues.
• Under oxidative stress, Chk2 promotes ATG7 ubiquitination by phosphorylating TRIM32 at Ser-55, positively regulating autophagosome assembly. Related references: PMID:37943659
• Chk2 also mediates CCAR2-SIRT1 association, required for CCAR2-dependent SIRT1 inhibition. Related references: PMID:25361978
• Keywords include serine/threonine-protein kinase, ATP-binding, nucleotide-binding, phosphoprotein, tumor suppressor, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen is a peptide from the N-terminal region (amino acids 1–100), so the antibody likely recognizes an epitope within this domain.
• For western blot (WB), expect bands at approximately 15–38 kDa (likely cleaved or isoform products) and 50–65 kDa (full-length and phosphorylated forms). Validate with appropriate positive and negative controls.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), nuclear staining is expected, with possible PML body localization.
• Consider optimizing antibody dilution for each application using relevant human cell lines or tissue samples.
• For ELISA, the antibody may be used as a capture or detection reagent; pair with a suitable secondary antibody and confirm performance with recombinant Chk2 protein.

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CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for targets like Chk2 in cancer, cell cycle, and DNA damage pathways. Whether you need proven rabbit monoclonal antibodies or rare-target sourcing support, we deliver end-to-end supply solutions from concept to clinic.