Anti-CHCHD1 Rabbit Polyclonal Antibody for WB and ELISA - Q96BP2

Core Product Specifications and Parameters

Biological Background: CHCHD1 Function and Localization

• CHCHD1, also known as small ribosomal subunit protein mS37 (MRP-S37), is a mitochondrial ribosomal protein encoded by the CHCHD1 gene (formerly C10orf34).
• It is a component of the mitochondrial 28S ribosomal subunit, essential for mitochondrial protein synthesis.
• Alternative names include coiled-coil-helix-coiled-coil-helix domain-containing protein 1 (reflecting the presence of a CHCH domain) and nuclear protein C2360, suggesting possible dual localization.
• Subcellular localization studies indicate CHCHD1 is primarily found in the mitochondrion, with additional detection in the nucleus.
• The protein contains disulfide bonds and has a calculated molecular weight of approximately 13.5 kDa (UniProt), consistent with the ~13 kDa band observed in western blotting.
• As a ribonucleoprotein component, CHCHD1 is part of the reference proteome and has been identified through proteomics identification efforts.
• It belongs to the mitochondrial ribosomal protein family, which is distinct from cytoplasmic ribosomal proteins and dedicated to translating the few mRNAs encoded by the mitochondrial genome.
• While no specific disease associations are reported, mitochondrial ribosomal proteins are critical for oxidative phosphorylation and cellular energy metabolism.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering the N-terminal region (amino acids 1–118), which is fully conserved in human CHCHD1; this region may target surface-exposed epitopes.
• As a polyclonal antibody, it recognizes multiple epitopes, potentially providing robust detection in denaturing western blots; however, lot-to-lot variability may occur, and optimization of working dilutions is recommended.
• For western blotting, a band near 13 kDa is expected; validate species reactivity in human and mouse samples, and consider using mitochondrial-enriched fractions to enhance signal.
• ELISA applications can be employed for quantifying CHCHD1 levels in cell or tissue lysates; cross-reactivity with mouse enables translational studies.
• Due to the nuclear localization reported for CHCHD1, consider testing the antibody in nuclear extracts if your experimental model requires it.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality antibodies like this anti-CHCHD1 polyclonal antibody, tailored for mitochondrial and ribosomal protein studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic. Partner with CamelBio to streamline your procurement and development workflow with reliable supply and expert technical support.