Anti-CD1d Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P11609 / P11610

Core Product Specifications and Parameters

Biological Background: CD1d Function and Localization

• CD1d is an antigen-presenting glycoprotein that belongs to the CD1 family of non-classical MHC class I-like molecules.
• It binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T (NKT) cells, playing a key role in innate and adaptive immunity.
• The protein is expressed on the surface of cortical thymocytes, certain T-cell leukemias, and in a variety of other tissues.
• Subcellularly, CD1d localizes to the cell membrane and traffics through endosomal and lysosomal compartments where it loads glycolipid antigens.
• Structurally, CD1d contains an immunoglobulin-like domain, disulfide bonds, and a transmembrane helix; it is synthesized with a signal peptide and undergoes glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 22-305) of mouse CD1d, suggesting the antibody likely recognizes the native conformation and may be suitable for detecting surface-expressed protein.
• For Western blotting, expect bands at approximately 38–39 kDa, consistent with the predicted molecular weights of CD1d1 and CD1d2.
• In immunofluorescence (IF-P), staining should appear predominantly at the plasma membrane and possibly in intracellular vesicular compartments; consider permeabilization for intracellular detection.
• ELISA applications may benefit from using recombinant CD1d protein or cell lysates; validate in the relevant sample system to confirm performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical services. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing support for rare targets such as CD1d. Whether advancing immunological assays or developing diagnostic kits, CamelBio streamlines your path from concept to clinic.