Anti-CHCHD3 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - Q9NX63

Core Product Specifications and Parameters

Biological Background: CHCHD3 Function and Localization

• Encoded by the CHCHD3 gene, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 (also known as Mic19) is a key component of the mitochondrial contact site and cristae organizing system (MICOS) complex.
• As a core MICOS subunit, CHCHD3 is crucial for maintaining crista junction architecture, inner membrane organization, and contact site formation with the outer membrane, thereby stabilizing the MICOS complex and preserving mitochondrial cristae morphology.
  Related references: PMID:25781180 PMID:32567732 PMID:33130824
• CHCHD3 also functions as a transcription factor that binds to the BAG1 promoter and represses BAG1 transcription.
  Related references: PMID:22567091
• The protein primarily resides in the mitochondrial inner membrane but can also localize to the cytoplasm and nucleus under certain conditions.
• Tissue expression is low in brain, placenta, lung, liver, kidney, and pancreas, but elevated in heart and skeletal muscle; higher levels are detected in primary lung cancers compared to normal lung tissue.
• Post-translational modifications include acetylation, phosphorylation, and myristoylation, and the protein contains disulfide bonds; it is classified as a lipoprotein and is involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–227 of human CHCHD3, which may allow detection of full-length and N-terminal isoforms across human, mouse, and rat samples.
• This polyclonal antibody has been validated in knockout models, confirming target specificity for Western blotting; always validate KO efficiency in your experimental system.
• For ELISA, consider using the recombinant immunogen protein as a coating antigen or reference standard.
• Optimize antibody dilution and incubation conditions for each application, as polyclonal lots can vary; a starting dilution of 1:500–1:2000 for WB and 1:1000–1:5000 for ELISA is typical but should be titrated.
• Note that the predicted molecular weight is 26 kDa; however, post-translational modifications or isoforms may result in additional bands.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supply partner, CamelBio streamlines your development pipeline by offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are investigating mitochondrial dynamics, cristae architecture, or cancer biomarkers, this anti-CHCHD3 antibody can be integrated into your diagnostic workflows. Contact our team to discuss your project's specific needs and take advantage of our comprehensive technical and logistical support.