Anti-Chk1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O14757

Core Product Specifications and Parameters

Biological Background: Chk1 Function and Localization

• Encoded by the CHEK1 gene, serine/threonine-protein kinase Chk1 undergoes alternative splicing to produce multiple isoforms, including an endogenous repressor isoform that antagonizes full-length Chk1 to promote the S to G2/M phase transition.
• Functions as a critical checkpoint kinase, required for cell cycle arrest and activation of DNA repair in response to DNA damage or unreplicated DNA, via recognition of the [R-X-X-S/T] substrate consensus sequence. Related references: PMID:11535615 PMID:12399544 PMID:12446774
• Phosphorylates CDC25A, CDC25B and CDC25C, creating 14-3-3 binding sites or priming them for NEK11-mediated polyubiquitination and degradation, thereby inhibiting CDK-cyclin complexes and blocking cell cycle progression. Related references: PMID:12676583 PMID:12676925 PMID:12759351 PMID:9278511 PMID:19734889 PMID:20090422
• Enhances DNA repair by homologous recombination through phosphorylation of RAD51 at Thr-309, promoting RAD51 release from BRCA2 and increasing its chromatin association. Related references: PMID:15665856
• Phosphorylates the C-terminus of TP53, enhancing its acetylation and transcriptional activity to promote cell cycle arrest and suppress proliferation. Related references: PMID:10673501 PMID:15659650 PMID:16511572
• Modulates chromatin structure and gene expression by phosphorylating histone H3.1 at Thr-11 (H3T11ph), leading to epigenetic silencing of a subset of genes (By similarity).
• Localizes to the nucleus, chromosomes, cytoplasm, and centrosome, reflecting its multifaceted roles in cell cycle control and genomic maintenance.
• Ubiquitously expressed with highest levels in thymus, testis, small intestine, and colon, tissues with high proliferation rates.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-100 at the N-terminus; consider confirming epitope specificity via peptide competition or deletion mutants in your assay system.
• For Western blotting, the expected molecular weight is ~54kDa; use a 10% SDS-PAGE gel and optimize transfer conditions for high-molecular-weight detection.
• In immunohistochemistry (paraffin), antigen retrieval with citrate buffer (pH 6.0) may be required; include thymus or testis as positive control tissues and rabbit IgG isotype as negative control.
• For ELISA, direct or sandwich formats can be evaluated; consider competitive assays with the immunizing peptide to validate binding specificity.
• This antibody is validated for human reactivity; cross-reactivity with other species has not been determined, so species-specific validation is recommended if extending to other models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, from validated monoclonal/polyclonal antibodies to bulk ancillary reagents. This anti-Chk1 antibody supports oncology and genomic integrity assay development, complementing our portfolio of optimized antibody pairs and rare-target sourcing capabilities. Leverage our technical consulting and supply chain expertise to accelerate your project from concept to clinic.