CHDH Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q8NE62

Core Product Specifications and Parameters

Biological Background: CHDH Function and Localization

• CHDH encodes choline dehydrogenase, a mitochondrial enzyme responsible for converting choline to betaine aldehyde, a key step in glycine betaine synthesis (By similarity).
• The protein localizes to both the mitochondrial inner membrane and outer membrane, where it participates in redox reactions.
• It is a flavoprotein that utilizes FAD as a cofactor, classifying it as an oxidoreductase.
• CHDH plays a role in mitochondrial quality control by mediating mitochondrial autophagy (mitophagy) following mitochondrial damage. Related references: PMID:25483962
• The primary sequence includes a transit peptide for mitochondrial import, and the mature protein is subject to acetylation.
• The molecular weight of the full-length precursor is approximately 65.4 kDa, consistent with the observed 65 kDa band in denaturing gels.
• CHDH is conserved across human, mouse, and rat, and antibodies targeting the immunogen region (aa 245–594) are expected to detect endogenous protein in these species.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal half of human CHDH, which may improve specificity and reduce cross-reactivity with other dehydrogenase family members.
• For western blotting, consider enriching mitochondrial fractions or using whole-cell lysates from metabolically active tissues (e.g., liver, kidney) to enhance detection.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), expect a mitochondrial staining pattern; co-stain with a mitochondrial marker to confirm localization.
• ELISA applications may benefit from titrating the antibody within the recommended range; validate against recombinant CHDH or cell lysates with known expression.
• Always include appropriate controls (e.g., CHDH knockout or knockdown cells) to verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to diagnostic and research needs. For CHDH-related projects, we can supply additional antibodies targeting mitochondrial metabolism and mitophagy pathways, along with rare-target raw-material sourcing. Our platform streamlines procurement from concept to clinic, ensuring consistent quality and supply continuity.