Anti-CHD4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14839

Core Product Specifications and Parameters

Biological Background: CHD4 Function and Localization

• CHD4 (Chromodomain-helicase-DNA-binding protein 4), also known as Mi2-beta, is an ATP-dependent chromatin remodeler that binds and distorts nucleosomal DNA. Related references: PMID:28977666 PMID:32543371
• It is a core component of the NuRD (Nucleosome Remodeling and Deacetylase) complex, which couples chromatin remodeling with histone deacetylation to regulate transcription. Related references: PMID:16428440 PMID:17626165 PMID:28977666
• CHD4 is recruited to DNA double-strand breaks via ZMYND8, where it facilitates transcriptional repression and promotes repair by homologous recombination. Related references: PMID:25593309
• The protein is implicated in neurogenesis, although the mechanistic details remain to be fully elucidated.
• CHD4 contains two chromodomains, a central ATPase/helicase domain, and multiple zinc-finger motifs, endowing it with DNA-binding and nucleosome remodeling activities.
• It localizes predominantly to the nucleus, but is also detected in the cytoplasm and at the centrosome/microtubule organizing center.
• Post-translational modifications including acetylation, phosphorylation, and ubiquitination may modulate CHD4’s stability, localization, and interactions.
• CHD4 is widely expressed across human tissues, reflecting its fundamental role in chromatin regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1520-1690 within the C-terminal region of human CHD4. This segment is conserved in mouse and rat, consistent with the reported cross-reactivity.
• For Western blot, expect a band at approximately 218 kDa. Use a low-percentage gel (e.g., 6-8%) and extended transfer times or wet transfer for high-molecular-weight proteins.
• Validate the antibody in your specific sample type and fixation conditions for IHC-P and IF/ICC. Antigen retrieval (e.g., citrate buffer pH 6.0) may enhance signal.
• As a polyclonal antibody, lot-to-lot variability is possible; include appropriate controls (e.g., siRNA knockdown or knockout lysates) to confirm specificity.
• For ELISA, optimize coating antigen and antibody dilution based on your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers your chromatin remodeling and DNA repair research with high-quality IVD raw materials like this CHD4 polyclonal antibody. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can procure rare-target raw materials to streamline your diagnostic development from concept to clinic.