Anti-CHD3 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q12873

Core Product Specifications and Parameters

Biological Background: CHD3 Function and Localization

• CHD3 is an ATP-dependent chromatin remodeling factor that directly binds and distorts nucleosomal DNA, facilitating changes in chromatin architecture. Related references: PMID:28977666
• As a core component of the nucleosome remodeling and deacetylase (NuRD) complex, CHD3 couples ATP hydrolysis to histone deacetylation, leading to transcriptional repression of target genes. Related references: PMID:16428440, PMID:28977666, PMID:30397230, PMID:27068747
• CHD3 localizes predominantly to the nucleus and PML bodies but also associates with the centrosome and cytoplasm, where it anchors pericentrin to maintain spindle organization and centrosome integrity. Related references: PMID:17626165
• The protein features multiple conserved domains, including two chromodomains, a SNF2-type ATPase/helicase domain, and PHD-type zinc fingers, which mediate chromatin targeting and protein interactions.
• Widely expressed across human tissues, CHD3 is implicated in neurodevelopment; mutations are associated with intellectual disability and other disease variants.
• Alternative splicing produces several isoforms, potentially expanding functional diversity in chromatin regulation.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 1–100 at the N‑terminus of human CHD3, a region predicted to be present in all known isoforms. Verify isoform detection in your specific model system.
• For Western blot, the predicted molecular weight is ~227 kDa; due to the large size, consider using low‑percentage (6–8%) SDS‑PAGE gels and extended transfer conditions to ensure efficient blotting.
• When performing immunofluorescence (IF‑P) or immunohistochemistry (IHC‑P), optimize fixation and permeabilization steps, as CHD3 localizes to both nuclear and cytoplasmic compartments.
• The antibody cross-reacts with mouse and rat CHD3; validate in appropriate tissue or cell lines for these species before quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with a comprehensive range of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. Our CHD3 monoclonal antibody serves epigenetics and chromatin biology research, and we facilitate rare target raw-material sourcing to streamline your assay development from concept to clinic.