Anti-CHD9 Polyclonal Antibody for WB and ELISA - Q3L8U1

Core Product Specifications and Parameters

Biological Background: CHD9 Function and Localization

• Known as ATP-dependent chromatin remodeler CHD9, also called Chromatin-related mesenchymal modulator (CReMM), CHD-9, or Kismet homolog 2 (KISH2).
• Functions as a transcriptional coactivator for PPARA and likely other nuclear receptors, promoting gene transcription.
• Possesses DNA-dependent ATPase activity and preferentially binds to A/T-rich DNA regions.
• Associates with A/T-rich regulatory regions in promoters of genes involved in osteogenic differentiation of progenitor cells (By similarity).
• Subcellular localization includes both cytoplasm and nucleus.
• Widely expressed at low levels, with restricted expression in bone marrow to osteoprogenitor cells adjacent to mature osteoblasts.
• Contains chromodomains, a helicase-like ATPase domain, and undergoes alternative splicing; post-translationally modified by acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• For Western blotting, note the large protein size (predicted ~326 kDa). Use low-percentage SDS-PAGE gels (e.g., 6% or gradient) and wet transfer with extended transfer time to ensure efficient transfer.
• The immunogen corresponds to amino acids 85–190, an N-terminal region; this may allow detection of full-length CHD9 and possibly N-terminal-containing isoforms.
• In ELISA, consider direct coating of recombinant CHD9 protein or peptide fragment for assay optimization.
• Verify antibody reactivity in your target species (human and mouse) with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, covering chromatin biology and transcriptional regulation research tools. We offer validated polyclonal and monoclonal antibodies, bulk reagent supply, and customized sourcing for rare targets like CHD9. Our portfolio supports diagnostic developers and research laboratories from concept to clinic.