Anti-CD97 Rabbit Monoclonal Antibody for WB, ELISA - P48960

Core Product Specifications and Parameters

Biological Background: CD97 (Adhesion G protein-coupled receptor E5) Function and Localization

• CD97, also known as Adhesion G protein-coupled receptor E5 or ADGRE5, is a member of the adhesion GPCR family characterized by a large extracellular domain with EGF-like repeats.
• The receptor is potentially involved in both adhesion and signaling processes early after leukocyte activation, playing an essential role in leukocyte migration.
• CD97 is broadly expressed on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes, as well as smooth muscle cells and various carcinomas.
• Subcellularly, CD97 localizes to the cell membrane and can also be found as a secreted form in the extracellular space.
• Expression is increased under inflammatory conditions, such as in the CNS of multiple sclerosis and in synovial tissue of rheumatoid arthritis, with detectable soluble CD97 in synovial fluid.
• The protein contains EGF-like domains, is glycosylated, and undergoes alternative splicing, with a molecular weight of approximately 92 kDa (predicted 91.9 kDa).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 21-398), which may detect both full-length membrane and soluble CD97 forms in WB.
• For WB, consider using reducing conditions and validate antibody specificity using appropriate controls, as CD97 may undergo glycosylation and alternative splicing.
• In ELISA, this antibody may be suitable for capturing soluble CD97 from serum or synovial fluid samples, but titration and validation in the relevant matrix are recommended.
• Confirm cross-reactivity with other species is not tested; thus, it is validated for human samples only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for diagnostic manufacturers and researchers, offering comprehensive access to IVD raw materials including validated anti-CD97 antibodies, recombinant proteins, and ancillary reagents. Whether you need optimized monoclonal antibodies for immunoassay development or bulk raw materials for large-scale production, CamelBio supports your programs from concept to clinic. For leukocyte adhesion and inflammation research, we provide tailored solutions to accelerate your diagnostic kit development.