[KO Validated] Anti-CHD1 Rabbit Polyclonal Antibody (pAb) for WB, IHC-P, ELISA - O14646

Core Product Specifications and Parameters

Biological Background: CHD1 Function and Localization

• CHD1 is an ATP-dependent chromatin-remodeling factor that acts as a substrate recognition component of the SAGA histone acetyltransferase complex, regulating RNA polymerase II transcription.
• It is also required for efficient transcription by RNA polymerase I, specifically the transcription termination step, and negatively regulates DNA replication.
• CHD1 helps maintain a specific chromatin configuration across the genome and is associated with histone deacetylase (HDAC) activity.
• The protein functions to bridge SNF2, FACT, PAF, and U2 snRNP complexes to trimethylated H3K4, modulating pre-mRNA splicing efficiency. Related references: PMID:18042460 PMID:28866611
• CHD1 is localized to both the nucleus and cytoplasm.
• It is widely expressed in many tissues, with highest levels in the brain, particularly the cerebellum and basal ganglia.
• CHD1 features chromodomains, a helicase ATP-binding domain, and a DNA-binding domain, and undergoes phosphorylation.
• Alternatively spliced isoforms exist.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C-terminal region (aa 1600 to C-terminus); consider this when designing detection strategies.
• Validate antibody specificity in your experimental system, as polyclonal antibodies may exhibit lot-to-lot variability.
• For Western blot, the predicted molecular weight is approximately 197 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• Cross-reactivity with mouse and rat samples is reported; validate in the relevant sample system.
• For IHC-P, optimize antigen retrieval and antibody dilution.
• As CHD1 is involved in chromatin remodeling and splicing, this antibody may be suitable for studying transcription-related processes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CHD1, a key chromatin remodeler implicated in transcription and splicing, we offer validated antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your epigenetic and transcription research. Partner with us for streamlined procurement and expert support in IVD development.