Anti-CD55 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P08174

Core Product Specifications and Parameters

Biological Background: Complement decay-accelerating factor (CD55) Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a 41kDa glycoprotein that belongs to the complement regulatory protein family and is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) moiety.
• Its primary function is to protect host cells from complement-mediated damage by binding to C4b and C3b fragments, preventing the formation and accelerating the decay of the C3 and C5 convertases, thereby inhibiting the complement amplification cascade. Related references: PMID:7525274 PMID:28657829
• CD55 serves as a receptor for several enteroviruses, including coxsackieviruses A21, B1, B3, B5, human enterovirus 70 and D68, and echoviruses 6, 7, 11, 12, 20, 21, facilitating viral entry into host cells.
• It is widely expressed on cell membranes of all cell types in contact with plasma complement proteins, including epithelial cells lining extracellular compartments, and is also present in a secreted form in body fluids.
• The CD55 protein contains four short consensus repeats (SCRs), also known as sushi domains, and features disulfide bonds that stabilize its structure; alternative splicing generates multiple isoforms.
• Genetic variants in CD55 are associated with CHAPLE syndrome (complement hyperactivation, angiopathic thrombosis, and protein-losing enteropathy), and the molecule carries the Cromer blood group antigen system.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50-150 of human CD55, located in the N-terminal region; this region is extracellular and suitable for detecting both native and denatured protein.
• For Western blotting, CD55 runs at approximately 41kDa under reducing conditions; use appropriate human cell lysates (e.g., HeLa, Jurkat) as positive controls and validate the band with known inhibitors or siRNA knockdown.
• In immunohistochemistry (IHC-P), optimize antigen retrieval (e.g., heat-induced epitope retrieval) and primary antibody dilution; tissues with high CD55 expression such as kidney, lung, or vascular endothelium are recommended.
• For ELISA, this monoclonal antibody can be employed as a capture or detection antibody; perform checkerboard titrations to determine optimal coating and detection concentrations.
• Since CD55 is GPI-anchored, consider using phosphatidylinositol-specific phospholipase C (PI-PLC) treatment to release the protein from the cell surface for functional assays or to confirm membrane localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a reliable single-source solution for IVD raw materials, supporting diagnostic developers and researchers from assay design to clinical readiness. With this anti-CD55 monoclonal antibody, you can further explore complement regulation or viral infection mechanisms, and we also offer validated antibody pairs, bulk reagents, and customized sourcing for rare or challenging targets. Partner with us to streamline your workflow and reduce time-to-market for your diagnostic kits.