CDC5L Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q99459

Core Product Specifications and Parameters

Biological Background: CDC5L Function and Localization

• CDC5L (Cell division cycle 5-like protein), also referred to as pombe cdc5-related protein, CDC5, CEF1, PCDC5RP, and CDC5-LIKE, is a human protein encoded by the CDC5L gene (Gene ID: 988).
• It acts as a DNA-binding protein involved in cell cycle control and may function as a transcription activator.
• CDC5L plays a central role in pre-mRNA splicing as a core component of precatalytic, catalytic, and postcatalytic spliceosomal complexes.
  Related references:
  PMID:11991638
  PMID:20176811
  PMID:28076346
• As a key subunit of the PRP19-CDC5L complex, it is required for activating pre-mRNA splicing and participates in the cellular DNA damage response (DDR).
  Related references:
  PMID:20176811
• CDC5L also functions within the minor spliceosome, contributing to the splicing of U12-type introns in pre-mRNAs.
• The protein localizes to the nucleus, nuclear speckles, and cytoplasm.
• CDC5L is a phosphoprotein and undergoes ubiquitination-like modifications (isopeptide bond, Ubl conjugation), as revealed by proteomic analyses.
• It is ubiquitously expressed in both fetal and adult tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning residues 300-400 of human CDC5L; thus, the antibody is expected to detect total CDC5L protein.
• For Western blot, a specific band at approximately 92 kDa should be observed; consider using nuclear extracts or whole cell lysates from human or mouse samples.
• In IHC-P, nuclear and nuclear speckle staining is anticipated; optimize antigen retrieval conditions and validate with appropriate tissue controls.
• When performing ELISA, direct or sandwich formats can be employed; titration of the antibody and validation with the immunogen peptide are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive source for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk auxiliary reagents. For research involving pre-mRNA splicing, cell cycle regulation, or DNA damage response, our anti-CDC5L monoclonal antibody offers a dependable reagent, and we can assist in procuring complementary targets or customized formulations. Partner with CamelBio to simplify your raw material procurement from concept to clinical application.