Anti-CD45RA Rabbit Monoclonal Antibody for IHC-P, ELISA - P08575-8

Core Product Specifications and Parameters

Biological Background: CD45RA Function and Localization

• CD45RA is isoform 6 of Receptor-type tyrosine-protein phosphatase C, also designated leukocyte common antigen (L-CA) and T200.
• Encoded by the PTPRC gene (CD45) in humans.
• The predicted molecular weight of this isoform is approximately 138 kDa.
• It is one of several alternatively spliced isoforms of the PTPRC gene, with isoform 6 specifically known as CD45RA.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 26–482 of the CD45RA isoform, corresponding to an extracellular region; this design suggests potential suitability for detecting surface-expressed CD45RA in immunohistochemistry on formalin-fixed paraffin-embedded (IHC-P) tissues.
• As a rabbit monoclonal antibody, consistent lot-to-lot performance can be expected; it is advisable to perform antibody titration and optimize detection conditions for each application.
• For IHC-P, antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required; validate the antibody in known human CD45RA-positive tissues such as tonsil or lymph node.
• In ELISA applications, the recombinant immunogen or native protein from lysates may be used; include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For CD45RA-related immunoassay development, we can assist in sourcing rare-target reagents and technical consultation, streamlining your transition from concept to clinical application.