Anti-ICAM-1/CD54 Rabbit Monoclonal Antibody for WB, IHC-P, FC, ELISA - Q00238

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (CD54) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, functioning as a cell adhesion molecule.
• It serves as a ligand for the integrins LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2), mediating leukocyte-endothelial cell adhesion and trans-endothelial migration.
• During leukocyte extravasation, ICAM-1 engagement stimulates endothelial apical cup assembly via ARHGEF26/SGEF and RHOG activation.
• In epithelial cells, ICAM-1 interaction with MUC1 promotes cell aggregation.
• ICAM-1 is localized to the cell membrane and features multiple immunoglobulin-like domains, a signal peptide, and a transmembrane helix.
• The protein undergoes post-translational modifications including N-linked glycosylation, disulfide bond formation, and ubiquitination, which may regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 28-493) of rat ICAM-1, encompassing multiple Ig-like domains, which suggests the antibody may recognize the native extracellular conformation; consider this for applications requiring native structure recognition (e.g., FC, ELISA).
• For Western blotting (WB), the predicted molecular weight is ~60 kDa; verify band specificity using appropriate lysates and loading controls.
• In immunohistochemistry (IHC-P), optimize antigen retrieval methods as ICAM-1 is a membrane protein; validate staining in relevant rat tissues known to express ICAM-1 (e.g., endothelium, inflamed tissues).
• For flow cytometry (FC), use indirect staining with appropriate secondary antibodies; confirm reactivity with rat cell lines or primary cells.
• ELISA applications may benefit from titration of both capture and detection antibodies to achieve optimal signal-to-noise ratios.
• Cross-reactivity is reported for rat; when using other species, validate by sequence alignment and pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

This anti-ICAM-1 antibody exemplifies CamelBio's commitment to supplying high-quality IVD raw materials. As a one-stop sourcing partner, we offer validated antibody pairs, monoclonal antibodies, bulk reagents, and customized raw-material solutions for leukocyte adhesion, inflammation, and beyond. Diagnostic manufacturers, labs, and research institutes can rely on our integrated technical services and consulting to accelerate projects from concept to clinic.