Anti-CD45 Rabbit Monoclonal Antibody for FC, ELISA - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) or T200, is a receptor-type tyrosine-protein phosphatase (PTPRC) that plays a critical role in immune cell signaling.
• It functions as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. Related references: PMID:35767951
• CD45 interacts with CLEC10A at the antigen-presenting cell–T cell interface, contributing to the modulation of T-cell activation thresholds and preventing autoreactivity.
• Alternatively spliced isoforms (ranging from isoform 1 to 8) are differentially expressed in thymocytes, with isoforms 1,2,3,5,7 detected and others not. Multiple protein weights (131–147 kDa) reflect this isoform diversity.
• Subcellularly, CD45 localizes to the cell membrane, membrane rafts, and the immunological synapse, consistent with its role in proximal T-cell receptor signaling.
• As a host factor, CD45 acts as a receptor for human cytomegalovirus protein UL11, mediating viral binding to T-cells and subsequent impairment of T-cell proliferation and signaling.
• Post-translational modifications include heavy glycosylation, phosphorylation, and the presence of a signal peptide, transmembrane helix, and tandem phosphatase domains.
• Broadly expressed on all nucleated hematopoietic cells, CD45 is essential for development and activation of lymphocytes, making it a key diagnostic marker for leukocyte identification.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 26–577) covers the extracellular region of human CD45, including multiple isoform-specific domains; this broad region suggests the antibody may recognize various CD45 isoforms in native conformations.
• For flow cytometry (FC), consider titrating the antibody on relevant human leukocyte subsets (e.g., PBMCs, Jurkat cells) to determine optimal staining concentrations; include isotype controls to assess nonspecific binding.
• For ELISA applications, evaluate direct or capture formats using recombinant CD45 protein or cell lysates; confirm linearity and sensitivity with standard curves.
• Since CD45 is heavily glycosylated, ensure that the antibody binding is not hindered by glycosylation in certain sample types; denaturing conditions may affect epitope accessibility.
• The antibody is supplied on ice; aliquot and store at recommended temperatures (e.g., -20°C) to maintain stability and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

As a premier provider of IVD raw materials, CamelBio supplies high-quality monoclonal antibodies like this anti-CD45 clone, along with validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare target proteins. Whether you are developing flow cytometry-based diagnostics or ELISA immunoassays for immune-mediated diseases, our integrated supply chain supports your transition from concept to commercialization. Partner with CamelBio for reliable access to critical immunological reagents and technical insights.