PE/Cyanine7 Rabbit anti-Human CD5 Monoclonal Antibody for Flow Cytometry - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and in a subset of B-cells known as B1a cells.
• It plays a central role in regulating TCR and BCR signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance.
• CD5 interacts with ligands CD5L and CD72 on B-cells, facilitating contact-mediated T-dependent B-cell activation and contributing to regulatory T and B-cell homeostasis.
• Functions as a negative regulator of TCR signaling by associating with LCK, CD3Z, PI3K, or CBL, and co-engagement with CD3 enhances CBL recruitment, leading to increased VAV1 phosphorylation and degradation. Related references: PMID:1384049 PMID:1385158 PMID:23376399
• In B-cells, CD5 modulates biology through ERK1/2 activation via a Ca(2+)-dependent pathway involving the TRPC1 channel, resulting in IL-10 production. Related references: PMID:27499044
• The protein localizes to the cell membrane and features a signal peptide, a single transmembrane helix, and multiple disulfide bonds and phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 25–371), suggesting recognition of native CD5 on live cells, making this antibody suitable for flow cytometry without permeabilization.
• As a PE/Cyanine7 conjugate, it can be directly used in multicolor panels; titrate the antibody to determine the optimal staining concentration for your specific sample type.
• Validate performance in the relevant human sample system (e.g., PBMCs) and include appropriate isotype and compensation controls.
• Avoid prolonged exposure to light to preserve fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering a comprehensive portfolio from validated monoclonal/polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. For T-cell biology and immunophenotyping research, products like this anti-CD5 antibody exemplify our commitment to supplying high-quality tools that accelerate assay development. Whether you need optimized antibody pairs or custom procurement, CamelBio streamlines your journey from concept to clinic.