Anti-CD5 Rabbit Monoclonal Antibody for FC, ELISA - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed on all T-cells and a subset of B-cells (B1a cells). It plays a critical role in regulating TCR and BCR signaling, thymocyte selection, and immune tolerance.
• CD5 functions as a negative regulator of TCR signaling during thymocyte development by associating with key signaling proteins including LCK, CD3Z chain, PI3K, and CBL.
  Related references:
  PMID:1384049
  PMID:1385158
• Co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment, leading to increased VAV1 phosphorylation and degradation.
  Related references:
  PMID:23376399
• CD5 mediates contact-dependent T-cell help to B-cells by binding ligands such as CD5L and CD72, contributing to T-dependent B-cell activation and regulatory T/B-cell homeostasis.
• In B-cells, CD5 modulates IL-10 production through ERK1/2 activation in a Ca(2+)-dependent pathway via TRPC1.
  Related references:
  PMID:27499044
• CD5 is a type I transmembrane glycoprotein localized to the cell membrane, characterized by extracellular scavenger receptor cysteine-rich (SRCR) domains and a cytoplasmic tail involved in signal transduction.

Experimental Guidance and Technical Tips

• The immunogen spans the majority of the extracellular region (aa 25-371), suggesting the antibody likely recognizes an epitope accessible on the cell surface, making it suitable for flow cytometry applications.
• For ELISA, consider using the recombinant CD5 protein as a standard to validate the assay's sensitivity and specificity.
• Optimize antibody titrations for each application and cell type, and include appropriate isotype controls for flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined source for high-quality IVD raw materials. For targets like CD5 involved in immune tolerance and T-cell development, CamelBio offers a range of monoclonal antibodies, recombinant proteins, and ancillary reagents to support your assay development and manufacturing needs. Whether you require validated antibody pairs, bulk quantities, or assistance sourcing difficult-to-find raw materials, CamelBio is your reliable partner from concept to clinic.