Anti-CD352/SLAMF6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96DU3

Core Product Specifications and Parameters

Biological Background: CD352/SLAMF6 Function and Localization

• SLAMF6 (CD352, NTB-A) is a self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family, modulating the activation and differentiation of NK, T, and B cells in both innate and adaptive immunity.
• In natural killer (NK) cells, SLAMF6 triggers cytolytic activity only when they express high surface densities of natural cytotoxicity receptors, signaling via VAV1 phosphorylation and requiring SH2D1B rather than SH2D1A. Related references: PMID:11489943, PMID:16920955
• SLAMF6 promotes T-cell differentiation into a Th17 phenotype, increasing IL-17 secretion; this costimulatory activity is dependent on SH2D1A. Related references: PMID:16920955, PMID:22184727
• Together with SLAMF1, SLAMF6 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage (by similarity).
• In the absence of SH2D1A/SAP, SLAMF6 can transmit negative signals to CD4+ T-cells and NKT cells, and it negatively regulates germinal center formation by inhibiting T-cell:B-cell adhesion (by similarity).
• SLAMF6 localizes to the cell membrane and contains immunoglobulin domains, disulfide bonds, and a transmembrane helix; it is a glycoprotein and phosphoprotein.
• Expressed on all (resting and activated) NK cells, T- and B-lymphocytes; increased surface expression is observed on T-cells of systemic lupus erythematosus (SLE) patients. Related references: PMID:11489943, PMID:22184727
• Alternative splicing yields isoforms; the product detects bands at 24 kDa and 37 kDa, reflecting potential isoforms or cleavage products in line with the predicted molecular weight of 37.3 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects bands around 24 kDa and 37 kDa; consider using freshly prepared lysates and loading controls to confirm specificity.
• In IHC-P, antigen retrieval may require optimization—test both heat-induced and enzymatic methods due to the transmembrane localization of the target.
• For ELISA, the antibody can serve as a capture or detection reagent; validate coating concentrations using the recombinant immunogen as a standard.
• The antibody is specific to human CD352; cross-reactivity with other species is not reported, so confirm in the relevant sample matrix when applied to non-human systems.

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