Anti-CDK5R1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q15078

Core Product Specifications and Parameters

Biological Background: CDK5R1 Function and Localization

• CDK5R1, also known as p35, p25, or p23, is the neuron-specific regulatory subunit of cyclin-dependent kinase 5 (CDK5).
• The p35/CDK5 complex is essential for neurite outgrowth and cortical lamination during brain development.
• It mediates dendritic spine morphogenesis through EFNA1-EPHA4 signaling.
• Participates in circadian clock regulation by modulating CLOCK protein phosphorylation and transcriptional activity.
• Subcellular localization includes cell membrane, neuron projections, nucleus, cytoplasm, and perinuclear region.
• Tissue expression is restricted to brain and neurons.
• Post-translational modifications include myristoylation for membrane anchoring, phosphorylation for activity regulation, and ubiquitination for protein turnover.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 34 kDa; however, cleavage products (e.g., p25) may appear at lower molecular weights, so validate in the relevant sample system.
• Immunofluorescence/immunocytochemistry may require permeabilization to detect intracellular targets; consider including appropriate controls for neuronal cell models.
• The immunogen is a synthetic peptide within amino acids 195-295, likely to recognize linear epitopes; this may be advantageous for ELISA and other denaturing applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, supporting diagnostic manufacturers and laboratories from early development to clinical application. This high-quality rabbit monoclonal CDK5R1 antibody is part of our expanding neuroscience portfolio, and we also provide validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need consistent lot-to-lot performance or tailored procurement solutions, CamelBio is your trusted partner for accelerating diagnostic innovation.