Anti-CD55 Rabbit Monoclonal Antibody for WB, ELISA - Q61475

Core Product Specifications and Parameters

Biological Background: Complement Decay-Accelerating Factor (CD55) Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored membrane protein that protects host cells from complement-mediated damage by inhibiting the formation and stability of C3/C5 convertases.
• It specifically binds to C4b and C3b fragments deposited on cell surfaces, preventing their interaction with complement components C2 and factor B, thereby blocking the amplification of the complement cascade.
• The protein contains multiple sushi (CCP) domains, is heavily glycosylated, and features disulfide bonds that stabilize its tertiary structure.
• CD55 is widely expressed in mouse tissues, including brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen, and lymph nodes.
• As a key regulator of innate immunity, CD55 prevents autologous tissue injury by restricting complement activation on self-cells.
• The GPI anchor localizes CD55 to lipid rafts on the cell membrane, facilitating its rapid interaction with complement proteins.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 35–361), suggesting the antibody likely recognizes the native protein structure; denaturing conditions may alter epitope accessibility.
• For Western blot, consider using reducing agents during sample preparation to resolve disulfide bonds; a predominant band near 42 kDa is expected.
• In ELISA, the antibody may detect recombinant or endogenous mouse CD55; validate optimal dilution and blocking conditions in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of validated antibodies, including this CD55 rabbit monoclonal antibody, tailored for complement research and immunoassay development. We offer one-stop access to IVD raw materials, from monoclonal/polyclonal antibodies and antigen pairs to ancillary reagents, streamlining your transition from concept to clinic. Partner with us to source high-quality reagents for your next diagnostic project.