Rabbit anti-Human CD55 Monoclonal Antibody for FC - P08174

Core Product Specifications and Parameters

Biological Background: CD55 Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored glycoprotein that regulates complement activation on host cells.
• It recognizes C4b and C3b fragments deposited on cell surfaces and disrupts the formation and stability of C3 and C5 convertases, thereby preventing complement-mediated damage. Related references: PMID:7525274, PMID:28657829
• CD55 is widely expressed on plasma membranes of cells that contact plasma complement, including epithelial and endothelial surfaces.
• The protein exists in multiple isoforms due to alternative splicing, with molecular weights ranging from 39 kDa to 59 kDa.
• Beyond its role in immunity, CD55 serves as a receptor for several enteroviruses, including coxsackieviruses A21, B1, B3, B5, and echoviruses 6, 7, 11, 12, 20, 21.
• Subcellularly, CD55 is found at the cell membrane and can be secreted into body fluids.
• Key structural features include a sushi domain repeat, disulfide bonds, and a GPI-anchor, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to for flow cytometry; consider including appropriate compensation controls when designing multicolor panels.
• The immunogen spans the N-terminal extracellular region (aa 35–353), making it suitable for detecting surface-expressed CD55; confirm expression in your cell system of interest.
• Due to the presence of multiple isoforms, verifying specificity with relevant controls or additional methods may strengthen your experimental conclusions.
• For optimal staining, titrate the antibody to determine the best concentration for your specific sample type and instrument.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for IVD development. For targets like CD55, which is central to complement regulation and innate immunity, our sourcing capabilities support your assay development from concept to clinic. Contact us to explore tailored raw-material solutions and technical consulting that streamline your pathway to market.